Neural crest-derived cells possess differentiation potential to keratinocytes in the process of wound healing - 16/01/22
, Tetsuo Suzawa d, Masahiro Chatani b, c, Megumi Ikeda b, c, e, Nobuhiro Sakai b, c, Yuki Azetsu b, c, Masahiro Takahashi a, Eri Urano f, Ryutaro Kamijo d, Koutaro Maki a, Masamichi Takami b, c, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 12 |
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Abstract |
Neural crest-derived cells (NCDCs), which exist as neural crest cells during the fetal stage and differentiate into palate cells, also exist in adult palate tissues, though with unknown roles. In the present study, NCDCs were labeled with EGFP derived from P0-Cre/CAG-CAT-EGFP (P0-EGFP) double transgenic mice, then their function in palate mucosa wound healing was analyzed. As a palate wound healing model, left-side palate mucosa of P0-EGFP mice was resected, and stem cell markers and keratinocyte markers were detected in healed areas. NCDCs were extracted from normal palate mucosa and precultured with stem cell media for 14 days, then were differentiated into keratinocytes or osteoblasts to analyze pluripotency. The wound healing process started with marginal mucosal regeneration on day two and the entire wound area was lined by regenerated mucosa with EGFP-positive cells (NCDCs) on day 28. EGFP-positive cells comprised approximately 60% of cells in healed oral mucosa, and 65% of those expressed stem cell markers (Sca-1+, PDGFRα+) and 30% expressed a keratinocyte marker (CK13+). In tests of cultured palate mucosa cells, approximately 70% of EGFP-positive cells expressed stem cell markers (Sca-1+, PDGFRα+). Furthermore, under differentiation inducing conditions, cultured EGFP-positive cells were successfully induced to differentiate into keratinocytes and osteoblasts. We concluded that NCDCs exist in adult palate tissues as stem cells and have potential to differentiate into various cell types during the wound healing process.
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Highlights |
• | Neural crest-derived cells (NCDCs) in palate mucosa tissues of adult mice exist as keratinocytes and stem cells. |
• | NCDCs were observed as keratinocytes in repaired mucosa in wound healing process. |
• | Palate mucosa NCDCs showed predominant multiplication as well as proliferation into stem cells in stem cell growth medium. |
• | With proliferation, NCDCs demonstrated an ability to differentiate into keratinocytes and osteoblasts. |
Abbreviations : AA, ALP, BMP-2, CK, Col1, Col1a1, Dex, EGFP, ICC, NCCs, NCDCs, PDGFR, PET, P0, P0-EGFP, Runx2, Sca-1, Snail, SOX2, SSEA3, Twist, β-GP
Keywords : Neural crest-derived cell, Palate mucosa, Keratinocyte, Osteoblast, Palate wound healing
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Vol 146
Article 112593- février 2022 Retour au numéro
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