Renal-protective effects of n-hexane layer from morning glory seeds ethanol extract - 14/11/17
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Highlights |
• | The present study aimed to evaluate protective effect of MGs-Hx on cisplatin-induced nephrotoxicity. |
• | MGs-Hx inhibited BUN, creatinine and uric acid levels in cispaltin-induced renal injury mice. |
• | MGs-Hx attenuated the cisplatin-induced inflammation and apoptosis in HEK-293 cells. |
• | Renoprotective effects of MGs-Hx were accompanied by suppression of MAPKs. |
Abstract |
Nephrotoxicity is a main problem in cancer patients using cisplatin. Oxidative stress, inflammation and apoptosis are the important mechanisms of cisplatin induced nephrotoxicity. In the present study, we investigated the effect of the extracts of morning glory on nephrotoxicity by cisplatin in human embryonic kidney cells 293 (HEK-293) and mice. Previous studies have reported that morning glory extracts showed potent activity on anti-inflammatory and anti-oxidant. However, the protective effects of the n-hexane layer of morning glory seed (MGs-Hx) on nephrotoxicity and its mechanisms have not been clearly understood. Oral administration with MGs-Hx showed protective effects in vivo experiments test and the treatment of MGs-Hx in a concentration of 100mg/kg/day had significant effect both of decreasing serum creatinine, BUN, serum uric acid level and reduced iNOS, COX-2 mRNA expressions with low side-effect. Moreover, cell viability was restored by MGs-Hx treatment compared to cisplatin-induced nephrotoxic HEK-293 cells. Co-treatment with MGs-Hx and cisplatin showed the significant effect to reduce inflammatory enzyme, iNOS expression and continuous production of NO. In addition, it exhibited a tendency to decreasing expression of apoptosis-related proteins, caspase-3, 8 and 9, and NF-κB translocation to nucleus as well as phosphorylation of p38, JNK, ERK in cisplatin-induced nephrotoxic HEK-293 cells. Our study provides insight into the underlying mechanisms of MGs-Hx and suggests that MGs-Hx might be a potential therapeutic agent to modulate inflammation and apoptosis in nephrotoxicity.
Le texte complet de cet article est disponible en PDF.Abbreviations : MGs-Hx, HEK-293, iNOS, COX-2, NO, NF-κB, IκBα, MAPKs, JNK, ERK
Keywords : Morning glory seed, Nephrotoxicity, Apoptosis, Inflammation, Human embryonic kidney cells 293, Cisplatin
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Vol 95
P. 1661-1668 - novembre 2017 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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