Active HCV infection is associated with increased circulating levels of interferon-gamma (IFN-?)-inducible protein-10 (IP-10), soluble CD163 and inflammatory monocytes regardless of liver fibrosis and HIV coinfection - 01/12/17
, Miriam Lichtner a, b , Paola Zuccalà a, Serena Vita a, Tiziana Tieghi a, b, Raffaella Marocco a, b, Stefano Savinelli a, Raffaella Rossi a, Marco Iannetta a, Michela Campagna a, Francesco Schiavone c, d, Fabio Mengoni a, Gianluca Russo a, Claudio Maria Mastroianni a, b, Vincenzo Vullo aBienvenue sur EM-consulte, la référence des professionnels de santé.
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Graphical abstract |
Highlights |
• | IP-10, sCD163 and sCD14 play an important role in the pathogenesis of HCV and HIV infection. |
• | IP-10 and sCD163 plasma levels were found increased in HCV monoinfected and HCV/HIV coinfected patients with active HCV replication (AHI). |
• | sCD14 plasma levels were found increased only in HIV monoinfected patients and HCV infected patients with cirrhosis. |
• | IP-10 levels were higher also in HCV positive subjects with low liver fibrosis stage, indicating an early inflammatory milieu. |
• | IP-10 levels strongly correlated with sCD163 suggesting a synergistic role in inflammation, while sCD14 plasma levels seem to be independent from sCD163 and IP-10 plasma concentrations. |
• | Active HCV infection is associated with a strong inflammatory state, even in the initial stage of liver fibrogenesis and regardless of HIV coinfection, thus underlying the need of an early antiviral treatment for HCV. |
Summary |
Background and objective |
Interferon-gamma (IFN-γ)-inducible protein-10 (IP-10), soluble (s) CD163 and sCD14 play an important role in the pathogenesis of HCV and HIV infection and are involved in inflammation and liver fibrosis. The aim of the present study was to evaluate at a single time point, plasma soluble biomarkers and inflammatory monocytes subsets in different groups of subjects: (i) HIV monoinfected patients on suppressive ART; (ii) HIV/HCV coinfected patients on ART, with undetectable HIV viremia (including either subjects who had active HCV replication or those who cleared HCV); (iii) HCV monoinfected individual with active viral replication.
Methods |
Hundred and twenty-nine plasma samples were analyzed including HCV and HIV monoinfected patients, HIV/HCV coinfected patients, with active HCV infection (AHI) or with HCV viral clearance (VHC) and healthy donors (HD). Levels of IP-10, sCD163 and sCD14 were measured by ELISA. Absolute cell counts of monocyte subpopulations were enumerated in whole blood by using flow cytometric analyses.
Results |
IP-10 and sCD163 plasma levels were higher in HCV monoinfected and in AHI coinfected pts compared to HIV monoinfected and HD, whereas sCD14 levels were higher only in HIV monoinfected patients. Considering the degree of fibrosis, sCD163 and sCD14 levels positively correlated with kPa values (as assessed by fibroscan) and FIB-4 in HCV monoinfected group. On the other hand, IP-10 did not correlate with the fibrosis stage and it was found increased also in patients with low fibrosis. Moreover, we found an increase of the inflammatory NCM subset, in non-cirrhotic HCV subjects, while no alterations were observed in HIV, AHI and VHC.
Conclusions |
Our study suggests a scenario in which active HCV infection is associated with a strong pro-inflammatory state, even in the initial stage of liver fibrosis, regardless the presence of HIV coinfection, thus underlying the need of an early anti-HCV treatment.
Le texte complet de cet article est disponible en PDF.Keywords : Hepatitis C virus (HCV), Human immunodeficiency virus (HIV), IP-10, sCD163, sCD14, Monocyte subsets
Abbreviations : HIV, HCV, ART, IP-10, sCD163, sCD14, AHI, VHC, HD, AST, ALT, PLT, BMI
Plan
Vol 41 - N° 6
P. 644-655 - décembre 2017 Retour au numéro
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