Idiopathic atrophoderma of Pasini and Pierini: A case study of collagen and elastin texture by multiphoton microscopy - 27/12/17
Abstract |
Background |
The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy.
Objective |
To identify histologic features that distinguish IAPP from unaffected skin.
Methods |
Eleven patients were included for conventional analyses. Masson trichrome– and Unna-Tanzer orcein–stained sections were evaluated using automated morphometry. Hematoxylin-eosin–stained sections were analyzed by multiphoton imaging using 2-photon excited fluorescence and second harmonic generation.
Results |
No abnormalities were found under light microscopy or by automated quantification. Multiphoton imaging revealed no difference in optical density of either collagen or elastic fibers in lesioned and unaffected skin; however, horizontal collagen fiber organization in lesion specimens increased toward the lower dermis, whereas elastic fibers featured greater disorganization within the upper dermis.
Limitations |
The low number of patients evaluated.
Conclusion |
The atrophic appearance of IAPP lesions reflects changes in organization, but not in collagen and elastic tissue content. Minute organizational differences that are imperceptible to the experienced pathologist and undetectable by automated analyses were revealed by multiphoton analyses, particularly second harmonic generation, in association with texture analyses.
Le texte complet de cet article est disponible en PDF.Key words : atrophoderma, atrophy, collagen fibers, elastic fibers, idiopathic atrophoderma of Pasini and Pierini, morphometry, pathology, scleroderma, SHG, second harmonic generation, TPEF, 2-photon excited fluorescence
Abbreviations used : AR, FFT, GLCM, H&E, IAPP, ROI, ROI 1, ROI 2, ROI 3, SHG, TPEF
Plan
Funding sources: This study was funded by the São Paulo Research Foundation (08/57906–3), the National Council for Scientific and Technological Development (573913/2008–0), and the Biologia das Doenças Neoplásicas da Medula Óssea (FAPESP grant 11/51959-0) project with fellowship support from CNPq (310518/2015-6). |
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Conflicts of interest: None declared. |
Vol 77 - N° 5
P. 930-937 - novembre 2017 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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