Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles - 04/04/18

Doi : 10.1016/j.biopha.2018.02.069

A.P.C. Ribeiro ^a, S. Anbu ^a,^b, E.C.B.A. Alegria ^a,^c,^⁎ , A.R. Fernandes ^a,^d, P.V. Baptista ^d,^⁎ , R. Mendes ^d, A.S. Matias ^d, M. Mendes ^c, M.F.C. Guedes da Silva ^a, A.J.L. Pombeiro ^a
^a Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
^b School of Chemistry, University of Birmingham, Edgbaston, B15 2TT, Birmingham, United Kingdom
^c Chemical Engineering Departament, Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, 1959-007 Lisboa, Portugal
^d UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Campus de Caparica, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal

^⁎Corresponding author: UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Campus de Caparica, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.UCIBIODepartamento Ciências da VidaFaculdade de Ciências e TecnologiaCampus de CaparicaUniversidade Nova de LisboaCaparica2829-516Portugal^⁎⁎Corresponding author at: Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.Centro de Química EstruturalInstituto Superior TécnicoUniversidade de LisboaAv. Rovisco PaisLisboa1049-001Portugal

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Abstract

Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO₃ by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV–vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC₅₀ (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC₅₀ 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

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Keywords : Green chemistry, Silver nanoparticles (AgNPs), DNA interaction, Cytotoxicity, Cancer cell lines, BSA interaction

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Vol 101

P. 137-144 - mai 2018 Retour au numéro

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Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles - 04/04/18

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