An in vitro comparative study of the antioxidant activity and SIRT1 modulation of natural compounds - 04/04/18
, Simona Daniele c, Silvia Pellegrini a, Claudia Martini c, Fabio Galetta a, Luca Giovannini b, Ferdinando Franzoni aBienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 15 |
| Iconographies | 9 |
| Vidéos | 0 |
| Autres | 0 |
Highlights |
• | Dietary antioxidants (polyphenols and berberine), SIRT1/AMPK and oxidative stress. |
• | Evaluate direct and specific antioxidant activity. |
• | Investigate the effect on SIRT1 expression and AMPK activation. |
• | No statistically significant decrease in the number of viable cells was found. |
Abstract |
Oxidative stress arises from an imbalance between the production of free radicals and antioxidant defences. Several studies have suggested that dietary antioxidants (such as polyphenols and berberine) may counteract oxidative stress through the involvement of the Sirtuin 1/Adenosine Monophosphate-Activated Protein Kinase (SIRT1/AMPK) pathway. The aim of this study was to evaluate the direct and specific antioxidant activity of some natural compounds, as well as their ability to modulate the expression of SIRT1 and the activation of AMPK.
Quercetin, tyrosol, ferulic acid, catechin, berberine and curcumin were evaluated for their specific and direct antioxidant activity with TOSC assay. Their ability to modulate SIRT1 and AMPK was assessed by immunoblotting assay, while their cytotoxicity by CellTiter-Blue Cell Viability Assay.
No statistically significant decrease (p > 0.05) in the number of viable cells was found upon challenging with the natural compounds. Quercetin exhibited the highest antioxidant activity against peroxyl radical and peroxinitrate derivates, while curcumin showed the best anti-hydroxyl activity with respect to the other compounds and, most importantly, respect to the reference antioxidants. Finally, all the tested compounds significantly increased the SIRT1 expression and the activation of AMPK.
Our results clearly disclose the specific antioxidant activity of these natural compounds and their ability to increase SIRT1 expression and AMPK activation.
Le texte complet de cet article est disponible en PDF.Abbreviations : AMPK, APAB, BSA, CCL4, DMEM, DMSO, DTPA, FBS, GSH, GSSG, HRP, KMBA, mTOR, NAD+, NAMPT, PBS, PMSF, PVDF, RONS, ROS, SDS, SIN-1, SIRT1, TOSC, T-TBS
Keywords : Oxidative stress, Antioxidants, Polyphenols, Berberine, SIRT1, AMPK
Plan
Vol 101
P. 805-819 - mai 2018 Retour au numéro
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