Swertia mussotii extracts induce mitochondria-dependent apoptosis in gastric cancer cells - 11/06/18
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Highlights |
• | Three fractions from S. mussotii extract were screened for in vitro anti-proliferative activity using MTT assay. |
• | Our results indicate that SMF70 induces apoptosis of MGC-803 cells via a mitochondrial-dependent pathway. |
• | Our findings demonstrate that S. mussotii extracts could be a potential new alternative therapeutic agent gastric cancer. |
Abstract |
Swertia mussotii (Gentianaceae) is a traditional Chinese medicinal plant grown in the Qinghai-Tibet Plateau. Three fractions from S. mussotii extract, named SWF50, SWF 70 and SWF100, were screened for in vitro anti-proliferative activity on two gastric cancer cell lines, MGC-803 and BGC-823 cells using MTT assay. Our results demonstrated that SMF70 showed an anti-proliferative effect in MGC-803 cells and SMF100 showed an anti-proliferative effect in BGC-823 cells in vitro. Moreover, both two fractions induced apoptosis via depolymerization of cytoskeletal filaments, increased cytoplasmic levels of ROS and Ca2+ and disrupted mitochondrial transmembrane potential. In addition, flow cytometry analysis indicated that both two fractions could induce cell apoptosis and arrest the cell cycle at S phase. Our results indicate that SMF70 induces apoptosis of MGC-803 cells and SMF100 induces apoptosis of BGC-823 cells via a mitochondrial-dependent pathway. Meanwhile, we also investigated antitumor effect of SMF70 in vivo, and exhibited effective tumor growth inhibition. Our findings demonstrate that S. mussotii extracts could be a potential new alternative therapeutic agent gastric cancer.
Le texte complet de cet article est disponible en PDF.Keywords : Swertia mussotii, Anti-Proliferative, Apoptosis, Cell cycle, Mitochondrial-Dependent
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Vol 104
P. 603-612 - août 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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