MiR-206 inhibits epithelial ovarian cancer cells growth and invasion via blocking c-Met/AKT/mTOR signaling pathway - 11/06/18
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Graphical abstract |
In this study, we found the expression of miR-206 was significantly down-regulated in epithelial ovarian cancer. Downregulation of miR-206 was associated with tumor metastasis and poor prognosis. In epithelial ovarian cancer cell lines, overexpression of miR-206 inhibited cancer cell proliferation and metastasis, and induced cell apoptosis via targeting c-Met and inhibiting the c-Met/AKT/mTOR signaling pathway.
Highlights |
• | Downregulation of miR-206 was associated with metastasis and poor prognosis. |
• | MiR-206 regulates cell cycle, cell apoptosis, and cancer cell metastasis. |
• | MiR-206 targets c-Met and blocks c-Met/AKT/mTOR signaling pathway. |
• | MiR-206 mimics inhibits epithelial ovarian tumor cells growth. |
Abstract |
Background |
MicroRNAs play important roles in the pathogenesis of various kinds of tumors. However, there are few studies on the expression profile and function of miRNAs in epithelial ovarian cancer. In this study, we performed microRNA array to compare the expression profile of microRNA in ovarian cancer tissues with noncancerous tissues.
Methods |
qRT-PCR was used to further confirm the microRNA expression levels in epithelial ovarian cancer tissues and cell lines. The function of microRNA was analyzed by overexpressing microRNA mimics followed by the analysis of cell cycle, proliferation, and metastasis. The downstream target of miR-206 was found and western blot analysis was performed to measure the activation of the downstream signaling pathway.
Results |
In this study, we found the expression of miR-206 was significantly down-regulated in epithelial ovarian cancer tissues and epithelial ovarian cancer cell lines. In epithelial ovarian cancer patients, downregulation of miR-206 was associated with metastasis and poor prognosis. In epithelial ovarian cancer cell lines, miR-206 contributed to the cell cycle regulation, cell apoptosis, and cancer cell metastasis. MiR-206 mimics inhibited cancer cell proliferation and metastasis, and induced cell apoptosis. Moreover, our results demonstrated that miR-206 directly targeted c-Met and repressed the activation of downstream AKT/mTOR signaling pathway.
Conclusion |
Our results demonstrated that miR-206 was down-regulated in epithelial ovarian cancer tissues and cell lines. MiR-206 inhibits the development of epithelial ovarian cancer cell by directly targeting c-Met and inhibiting the c-Met/AKT/mTOR signaling pathway.
Le texte complet de cet article est disponible en PDF.Keywords : MiR-206, c-Met, AKT/mTOR, Epithelial ovarian cancer, MicroRNA profiling
Plan
Vol 104
P. 763-770 - août 2018 Retour au numéro
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