Morphometric changes and AQP2 expression in kidneys of young male rats exposed to chronic stress and a high-sucrose diet - 11/07/18
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Graphical abstract |
Highlights |
• | Chronic stress in young rats generates nephrological changes. |
• | High-sucrose diet prevents low creatinine levels in chronic stress via renal AQP-2. |
• | Chronic stress plus high-sucrose has similar kidney effects as stress alone. |
• | Chronic stress may generate an accelerated glomerular hypertrophy before puberty. |
Abstract |
Objective |
Consumption of a cafeteria-like diet and chronic stress have a negative impact on kidney function and morphology in adult rats. However, the interaction between chronic restraint stress and high-sucrose diet on renal morphology in young rats is unknown. A high-sucrose diet does not modify serum glucose levels but reduces serum corticosterone levels in stressed young rats, in this way it is confusing a possible potentiate or protector effect of this diet on kidney damage induced by stress.
Methods |
Wistar male rats at 4 weeks of age were randomly assigned into 4 groups: control (C), stressed (St), high-sucrose diet (S30), and chronic restraint stress plus a 30% sucrose diet (St + S30). Rats were fed with a standard chow and tap water (C group) or 30% sucrose diluted in water (S30 group). Chronic restraint stress consisted of 1-h daily placement into a plastic cylinder, 5 days per week, and for 4 weeks.
Results |
Stressed rats exhibited a low number of corpuscles, glomeruli, high number of mesangial cells, major deposition of mesangial matrix and aquaporin-2 protein (AQP-2) expression, and low creatinine levels. Meanwhile, high-sucrose diet ameliorated AQP-2 expression and avoided the reduction of creatinine levels induced by chronic stress. The combination of stress and high-sucrose diet maintained similar effects on the kidney as stress alone, although it induced a greater reduction in the area of proximal tubules.
Conclusions |
Our results show that both chronic stress and a high-sucrose diet induce histological changes, but chronic stress may generate an accelerated glomerular hypertrophy associated with functional changes before puberty.
Le texte complet de cet article est disponible en PDF.Keywords : Creatinine, Mesangial cell, Mesangial matrix, Puberty rats, Restriction stress, High sucrose diet
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Vol 105
P. 1098-1105 - septembre 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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