Oncogenic miR-663a is associated with cellular function and poor prognosis in renal cell carcinoma - 11/07/18
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Highlights |
• | MiR-663a is present at significantly higher level in RCC tissue and RCC cell lines. |
• | Upregulation of miR-663a promotes RCC cell migration, invasion and proliferation, and inhibits apoptosis. |
• | Patients with high expression of miR-663a have a remarkable shorter overall survival. |
• | Oncogenic miR-663a is associated with cellular function and poor prognosis in renal cell carcinoma. |
Abstract |
Background |
MicroRNA(miRNA) plays a key regulatory role in various stages of tumorigenesis, including cell growth, cell cycle control, apoptosis avoidance, tissue invasion, and metastasis. Several microRNAs are involved in the development of renal cell carcinoma (RCC) and the malignant transformation process. However, the effects of miR-663a on RCC have rarely been reported.
Methods |
In the present study, the expression of miR-663a was examined in RCC using matched normal kidney tissues and four cell lines (786-O, Caki-1, ACHN and HK-2). MicroRNA mimics were transiently transfected into RCC cells and the effects of over expression on proliferation, migration, invasion, and apoptosis was observed. In addition, the relationship between miR-663a expression in 42 formalin-fixed paraffin-embedded (FFPE) clear cell renal carcinoma (ccRCC) samples and clinical pathological variables and overall survival was investigated. We evaluated the prognostic value of miR-663a expression in ccRCC by experimental results.
Results |
The results showed that the expression of miR-663a was up-regulated in RCC cells and tissues and miR-663a was associated with proliferation, migration, invasion, and apoptosis of RCC. Cox proportional hazard regression analysis showed that a high expression of miR-663a patients had a significantly shorter overall survival in univariate and multivariate analysis. Kaplan-Meier survival curves showed that a high expression of miR-663a patients had a significantly shorter overall survival.
Conclusions |
These results indicate that miR-663a can be used as an independent marker for the poor prognosis of ccRCC, and may also play an important role as a tumor oncogene in the occurrence and development of RCC.
Le texte complet de cet article est disponible en PDF.Keywords : microRNA, miR-663a, Renal cell carcinoma, Function, Prognosis
Plan
Vol 105
P. 1155-1163 - septembre 2018 Retour au numéro
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