Umbilical cord blood-mesenchymal stem cells and carvedilol reduce doxorubicin- induced cardiotoxicity: Possible role of insulin-like growth factor-1 - 11/07/18
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Abstract |
In this study, we tried to demonstrate the effects of adding human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) to carvedilol in improving the doxorubicin- induced cardiotoxicity in rats. Rats were randomly divided into four groups: group 1: control group, group 2: doxorubicin untreated group, group 3: rats injected with doxorubicin and received carvedilol, and group 4: rats injected with doxorubicin and received carvedilol and stem cell–treated. Electrocardiography (ECG) was performed to assess cardiac function after animals were sacrificed. Cardiac muscle sections were examined histologically using H&E, Masson trichrome and immunohistochemically using caspase 3 immunostaining. The morphometric and statistical analysis was performed. Levels of malondialdehyde (MDA), superoxide dismutase (SOD), insulin-like growth factor (IGF-1), and vascular endothelial growth factor (VEGF) were measured. We concluded that combination of hUCB-MSCs and carvedilol markedly improves histological and immunohistochemical structure of cardiac muscle fibers and restores cardiac function in doxorubicin- induced cardiotoxicity in rats.
Le texte complet de cet article est disponible en PDF.Abbreviations : BM-MSCs, BNP, Caspases, CK-MB, DOX, ECG, ELISA, FACS, GVHD, HGF, hUCB-MSCs, I/R, IGF-1, MDA, MNCs, MNCs, NF-κB, ROS, SOD, UCSCs, VEGF
Keywords : Human umbilical cord blood mesenchymal stem cells, Cardiotoxicity, Doxorubicin, Carvedilol, IGF-1, caspase-3
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Vol 105
P. 1192-1204 - septembre 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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