LncRNA MEG3 enhances 131I sensitivity in thyroid carcinoma via sponging miR-182 - 11/07/18
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Graphical abstract |
The flow chart schematic model. 131I-resistant TC cells induce down-regulation of MEG3. Then, decreased MEG3 resulted in an increase of miR-182 expression. Finally, up-regulated miR-182 enhanced 131I resistance of TC cells by facilitating proliferation, as well as suppressing apoptosis and DNA damage.
Highlights |
• | MEG3 expression was down-regulated in 131I-resistant TC cells. |
• | MEG3 overexpression inhibited proliferation and induced apoptosis in 131I-resistant TC cells. |
• | MEG3 suppressed miR-182 expression as a molecular sponge. |
• | MEG3 exerted functions in 131I-resistant TC cells by regulating miR-182. |
Abstract |
Background |
Long non-coding RNA (LncRNA) MEG3 has been demonstrated as a tumor suppressor in various cancers, including thyroid carcinoma (TC). However, the detail functions and possible mechanisms of MEG3 in 131I resistance of TC remain to be uncovered.
Methods |
qRT-PCR was performed for the detection of MEG3 and miR-182 levels. 131I-resistant TC cells were constructed by continuous exposure to stepwise increased concentrations of 131I. Western blot assay was used to measure the protein expressions of γ-H2 AX and H2 AX. CCK-8 and flow cytometry assays were carried out for the evaluation of cell viability and apoptosis, respectively. Bioinformatics and dual-luciferse assays were conducted to prove the interaction of MEG3 and miR-182.
Results |
MEG3 expression was down-regulated in TC tumor tissues, and the cumulative survival rate was decreased in low MEG3 expression group in TC patients under 131I treatment. MEG3 expression appeared a decline and miR-182 expression displayed an increase in 131I-resistant FTC-133 (res-FTC-133) and TPC-1 (res-TPC-1) cells. Moreover, MEG3 overexpression suppressed 131I-resistant cell viability, promoted apoptosis and induced DNA damage. MEG3 was verified as a molecular sponge for miR-182, and inhibition of miR-182 exerted similar functions as MEG3 overexpression. Furthermore, MEG3 knockdown substantially abrogated the anti-cancer functions of anti-miR-182.
Conclusions |
MEG3 enhanced the radiosensitivity of 131I in TC cells via sponging miR-182, indicating that MEG3 may act as a potential biomarker and therapeutic target for TC patients with 131I resistance.
Le texte complet de cet article est disponible en PDF.Abbreviations : TC, FCM, lncRNAs, MEG3, miRNA, PTC, CHL1
Keywords : lncRNA, MEG3, Thyroid carcinoma131I radioactivity, miR-182
Plan
Vol 105
P. 1232-1239 - septembre 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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