Over-expression of miR-15a-3p enhances the radiosensitivity of cervical cancer by targeting tumor protein D52 - 11/07/18
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Graphical abstract |
Highlights |
• | miR-15a-3p is down-regulated in radiotherapy cervica tissues and cells. |
• | miR-15a-3p regulated the proliferation of Hela and SiHa cells exposed to radiation. |
• | TPD52 regulated radiation-induced Hela and SiHa cells proliferation and apoptosis. |
• | miR-15a-3p enhanced radiosensitivity of cervical cancer in xenograft models. |
Abstract |
Background |
Radioresistance is a challenge in the treatment of cervical cancer. Recent studies have reported that microRNAs (miRNAs) mediate radiotherapy resistance and play a vital role in the occurrence and development of cancer. The aim of this study was to investigate whether the expression of miR-15a-3p was correlated with radiosensitivity in cervical cancer.
Methods |
Quantitative real-time PCR experiment was performed to detect the expression of miR-15a-3p in cervical cancer tissues and cells lines. Then, the effect of miR-15a-3p on proliferation in cervical cancer cells radiation-induced were determined by using CCK-8, clonogenic formation and EdU assays. In addition, the TUNEL, flow cytometry analysis and western blotting assays were conducted to evaluate radiation-induced cells apoptosis. A dual-luciferase reporter assay was used to test the target. In addition, tumor xenograft experiment was conducted to test tumor growth in vivo.
Results |
In this study, miR-15a-3p was downregulated in cervical cancer tissues and cells lines, however, the expression of miR-15a-3p significantly increased exposed to radiation. Moreover, over-expression of miR-15a-3p inhibited cells proliferation and enhanced cells apoptosis radiation-induced. Further, TPD52 was identified as a direct target of miR-15a-3p. Inhibition of TPD52 could suppress cells proliferation and induce cells apoptosis. Tumor xenograft experiments indicated that over-expression of miR-15a-3p could increase sensitivity to radiation therapy by targeting TPD52.
Conclusion |
In conclusion, our findings suggested that miR-15a-3p enhanced radiosensitivity in cervical cancer by targeting tumor protein D52, suggesting that miR-15a-3p may be a potential therapeutic target for cervical cancer patients.
Le texte complet de cet article est disponible en PDF.Keywords : miR-15a-3p, Tumor protein D52, Radiosensitivity, Cervical cancer
Plan
Vol 105
P. 1325-1334 - septembre 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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