Polygonatum sibiricum rhizome promotes sleep by regulating non-rapid eye movement and GABAergic/serotonergic receptors in rodent models - 11/07/18
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Graphical abstract |
Highlights |
• | PSE significantly reduced latency time and increased total sleep in mice. |
• | PSE increased total sleep time by increasing NREM and decreasing REM in rat. |
• | PSE effectively restores caffeine-induced sleep disturbance. |
• | PSE showed sleep enhancement through GABAergic/serotonergic action. |
• | Glyceryl monolinoleate and Oleamide contributed to sleep-promoting effect of PSE. |
Abstract |
The aim of this study is to investigate the sleep-promoting effect of a water extract of the Polygonatum sibiricum rhizome (PSE) in rodent models. PSE contained oleamide (0.10 mg/g extract) and glyceryl monolinoleate (0.17 mg/g extract), which are recognized as sleep-promoting substances. In pentobarbital-induced sleep model at hypnotic level, PSE (160 mg/kg) administration significantly decreased sleep latency time by 29% (2.7 min) and increased sleep duration time by 70% (68.4 min) compared with the normal control (3.8 min and 40.7 min, respectively). In the electroencephalography (EEG) analysis of rats, PSE-mediated sleep promotion accompanied the change of sleep architecture including increase of non-rapid eye movement (NREM) and decrease of REM. This sleep promoting effect was more obvious in caffeine-induced awakening model; total sleep time was increased by 40% along with increased NREM by PSE treatment at 160 mg/kg. In addition, PSE significantly increased the protein and mRNA levels of GABAA-R2 and 5-HT1A receptor, the major sleep-related neurotransmitter receptors. Furthermore, glyceryl monolinoleate and oleamide effectively bound to GABAA receptor in a competitive binding assay. These results indicate that PSE-mediated sleep-promoting effect is associated with the extension of NREM and upregulation of GABAA-R2 and 5-HT1A, and is mediated by binding to the GABAA receptor in vertebrate models.
Le texte complet de cet article est disponible en PDF.Keywords : Polygonatum sibiricum, Insomnia, Sleep, Caffeine, γ-aminobutyric acid, Vertebrate
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Vol 105
P. 167-175 - septembre 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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