Dangguishaoyao-San attenuates LPS-induced neuroinflammation via the TLRs/NF-κB signaling pathway - 11/07/18
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Graphical abstract |
Highlights |
• | DSS attenuates BV2 microglia cells inflammation. |
• | DSS protect against neuroinflammation via TLR4/NF-κB signaling pathway. |
• | TLR4 plays an important role in neuroinflammation. |
Abstract |
Introduction |
Dangguishaoyao-San (DSS) is composed of six traditional Chinese medicines, including Angelica sinensis, Paeoniae radix, Rhizoma Ligusticum, Poria cocos, Rhizoma Atractylodis Macrocephalae, and Rhizoma Alismatis. DSS has been reported to be effective in alleviating the symptoms of Alzheimer’s disease (AD). The aim of this study was to investigate the mechanism of action of DSS in vitro using lipopolysaccharide (LPS)-stimulated BV-2 microglia cells.
Materials and methods |
BV-2 cells were pretreated with 0.58–1.16 mg/mL of DSS for 2 h and then treated with 1 μg/mL LPS for 24 h. Cell viability was determined by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The protein expression levels were measured by Western blots. Inflammatory factors were detected by enzyme-linked immunosorbent assays (ELISAs). The mRNA levels of inflammatory factors were analyzed by quantitative real-time PCR (qRT-PCR).
Results |
DSS treatment at concentrations of 0.58–1.16 mg/mL resulted in no significant cytotoxicity. DSS attenuated the release of pro-inflammatory factors, such as interleukin-1β (IL-1β), iNOS and tumor necrosis factor-α (TNF-α) in LPS-induced BV-2 cells. DSS attenuated the mRNA expression of pro-inflammatory cytokines, TLR2, and TLR4 and decreased TLR4 and TLR protein levels as well as the phosphorylation of IκB in LPS-induced BV-2 cells. DSS also down-regulated the nuclear translocation of p65.
Conclusion |
This study demonstrated that DSS has a protective effect on neuroinflammation in LPS-induced BV-2 microglia cells through the TLRs/NF-κB signaling pathway.
Le texte complet de cet article est disponible en PDF.Abbreviations : DSS, AD, LPS, Aβ, NF-κB, IκB, TLR-2, TLR-4, TNF-α, iNOS, IL-1β, DAPI, PBS, MTT
Keywords : Dangguishaoyao-San (DSS), BV-2 Microglia cell, LPS, Alzheimer’s disease
Plan
Vol 105
P. 187-194 - septembre 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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