Ellagic acid impedes carbontetrachloride-induced liver damage in rats through suppression of NF-kB, Bcl-2 and regulating Nrf-2 and caspase pathway - 11/07/18
, Ozlem Gok b, Orhan Erman b, Tuncay Kuloglu cBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 8 |
| Iconographies | 9 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | Ellagic acid reduced liver DNA damage in carbon tetrachloride-induced rats. |
• | Ellagic acid significantly inhibits NF-kB and Bcl-2 protein expression but it induces Nrf-2 and Caspase-3 protein expression. |
• | Regular ellagic acid consumption may protect liver against to oxidative damage. |
• | Ellagic acid increased catalase activity of liver in carbon tetrachloride-induced rats. |
• | Ellagic acid may be used as a drug for treatment in future. |
Abstract |
The use of natural antioxidants instead of conventional treatments is considered effective and safe alternative therapy for hepatotoxicity. Ellagic acid (EA) is a strong antioxidant matter having protecting effect particularly on the liver. Hepatotoxic compounds can cause very heavy damage. Among these chemical hepatotoxins, CCl4 are responsible for the trichloromethyl radical resulting from biotransformation of the liver. The aim of this study was to examine whether EA plays a protective role against to liver damage induced with carbon tetrachloride (CCl4) in rats. In this study, 36 male wistar albino (n = 36, 8 weeks old) rats were used. The rats were distributed into 4 groups, and 9 rats involved in each group. The groups were: (i) Control Group: Fed with standard diet; (ii) EA Group: Fed with standard diet + EA; (iii) CCl4 Group: Fed with standard diet + CCl4; (iv) CCl4 + EA Group: Fed with standard diet + CCl4 + EA. After 8 weeks, the rats were decapitated and the liver tissue were examined. As a result; EA application created a significant difference (p < 0.05) on caspase-3, bcl-2, NF-kB and Nrf-2 expression in the CCl4 + EA group in comparison to CCl4 group. Caspase-3 and Nrf-2 expression levels were increased in the CCl4 + EA group in comparison to CCl4 group, but bcl-2 and NF-kB expression levels were decreased. In TUNEL assay examinations, apoptotic index ratio was decreased in the CCl4 + EA group in comparison to CCl4 group. These results show that EA reduce liver damage ratio at wistar albino rats and also these results suggest that ellagic acid may be a potentially protective drug against to liver damage in future.
Le texte complet de cet article est disponible en PDF.Abbreviations : CCl4, EA, EDTA, FUDAM, ip, MDA, NF-kB, PAGE, PMSF, ROS, TGF-β1, TNF-α, LSD, SDS, AlCl3, H2O2, TUNEL, ConA, DEN, TBA
Keywords : Ellagic acid, Liver, Apoptosis, Caspase-3, Bcl-2, Nrf-2, NF-kB
Plan
Vol 105
P. 662-669 - septembre 2018 Retour au numéro
Article précédent
- α-Terpineol reduces cancer pain via modulation of oxidative stress and inhibition of iNOS
- Daniele Nascimento Gouveia, Janara Santos Costa, Marlange Almeida Oliveira, Thallita Kelly Rabelo, Ana Mara de Oliveira e Silva, Adriana Andrade Carvalho, Rodrigo Miguel-dos- Santos, Sandra Lauton- Santos, Luciana Scotti, Marcus Tullius Scotti, Márcio Roberto Viana dos Santos, Lucindo José Quintans-Júnior, Ricardo Luiz Cavalcanti De Albuquerque Junior, Adriana Gibara Guimarães
- MicroRNA-302c represses epithelial–mesenchymal transition and metastasis by targeting transcription factor AP-4 in colorectal cancer
- Wenqi Ma, Bailing Liu, Jie Li, Jue Jiang, Ru Zhou, Lili Huang, Xiaopeng Li, Xin He, Qi Zhou
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?