In vitro comparison of endothelialisation process and biocompatibility of 3 percutaneous atrial septal defect devices using human endothelial cells - 14/08/18
, Zakaria Jalal 1, Audrey Aussel 2, 3, Noëlie Thebaud 2, 3, Martine Renard 4, Reine Bareille 2, Jean Ripoche 2, Marlène Durand 4, Jean-benoit Thambo 1, Laurence Bordenave 3, 4Bienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 2 |
| Iconographies | 5 |
| Vidéos | 0 |
| Autres | 0 |
Résumé |
Introduction |
The first cause of congenital heart disease is atrial septal defect (ASD). Several devices were developed to close secundum ASD but complications can occur (thromboembolic, infectious…) [1, 2, 3]. Providers claim different coatings supposed to improve device haemocompatibility.
Objective |
To study, in vitro, the ability of 3 devices to be endothelialized by endothelial cells derived from circulating human endothelial progenitors cells (EPCs), to induce platelet or complement activations or coagulation intrinsic pathway activation.
Methods |
EPCs from umbilical cord blood were extracted, cultured and characterized (CD31, VE-cadherin and von Willebrand factor). Devices were seeded with 100,000 cells/cm2. EPC adhesion, at 3 and 24hours, was investigated (biological activity of N acetyl β-D-hexosaminidase). EPC proliferation was monitored with Alamar blue® test which allowed a longitudinal follow-up (Days 1, 3, 6, 8, 10 and 12). C3a assay was performed after blood-contacting devices (standard ASTM F1984) [4]. Thereafter, platelet activation (via Pselectin and GPIIBIIA) and blood coagulation on biomaterials (standard ASTM F2888, F2382) [5, 6] were explored.
Results |
With regard to EPCs adhesion and proliferation (Fig. 1, Fig. 2), no statistically significant differences were found between 3 devices. There was a significant EPC proliferation on each device as a function of time appearing at Day 8 for devices 2 and 3 and Day 10 for device 1. No complement activation was detected by the C3a assay (device 1: 3584±978ng/mL, device 2: 3386±1092ng/mL and device 3: 4612±1657ng/mL) (Fig. 3). No platelet activation occurred within 15min of contact with devices (Figs 4 and 5). However, there was a minimal activation of coagulation for 3 devices (Mean time for PTT test on device 1: 183±23.3 s, device 2: 170.7±22.2 s and device 3: 167±22.6 s).
Conclusion |
Despite different coatings, the haemocompatibility of three devices was comparable. The benefit of effective anticoagulation should be confirmed by future clinical studies.
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Vol 10 - N° 3-4
P. 275-276 - septembre 2018 Retour au numéro
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