Differentially expressed circular RNAs in systemic lupus erythematosus and their clinical significance - 20/09/18
, Dong-Qing Ye b, c, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 8 |
| Iconographies | 5 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | Provide a expression profile of circRNAs in plasma of SLE patients. |
• | Verify the expression levels of circRNA both in plasma and PBMCs. |
• | Reveal the potential ceRNAs for differentially expressed circRNA. |
Abstract |
It has been reported that circRNAs are diff ;erentially expressed in many diseases and can be used as new biomarker to facilitate disease diagnosis. Circular RNAs (circRNAs) microarray were used to identify dysregulated circRNAs in plasma of systemic lupus erythematosus (SLE) patients. Then, we confirmed the microarray data by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in both plasma and peripheral blood mononuclear cells (PBMCs) of SLE. One hundred and twelve circRNAs were identified to dysregulated expressed in plasma of SLE as compared to healthy controls. The results of qRT-PCR showed that the levels of hsa_circRNA_407176 and hsa_circRNA_001308 were decreased in both plasma and PBMCs of SLE when compared with healthy controls. The receiver operating characteristic (ROC) curve area of hsa_circRNA_407176 and hsa_circRNA_001308 in plasma were 0.599 and 0.662, respectively. The area under the ROC curves of hsa_circRNA_407176, hsa_circRNA_406567 and hsa_circRNA_001308 in PBMCs were 0.806, 0.744, and 0.722, respectively. Our study illustrated that hsa_circRNA_407176 and hsa_circRNA_001308 in plasma and PBMCs could be potential biomarkers for SLE.
Le texte complet de cet article est disponible en PDF.Abbreviations : SLE, miRNA, lncRNA, circRNA, CDR1as, qRT-PCR, PBMCs, ceRNA, SLEDAI-2k, FC, FDR, ROC curve, AUC, anti-SSA, CRP, HCC, MSCs, NF-kB
Keywords : Circular RNA, Systemic lupus erythematosus, Microarray, Quantitative reverse transcription polymerase chain reaction
Plan
Vol 107
P. 1720-1727 - novembre 2018 Retour au numéro
Article précédent
- F-box and leucine-rich repeat protein 5 promotes colon cancer progression by modulating PTEN/PI3K/AKT signaling pathway
- Hui Yao, Song Su, Dong Xia, Mingming Wang, Zonglin Li, Wangsheng Chen, Lei Ren, Liang Xu
- Knockdown of LOXL1 inhibits TGF-β1-induced proliferation and fibrogenesis of hepatic stellate cells by inhibition of Smad2/3 phosphorylation
- Li Ma, Yanli Zeng, Junfeng Wei, Dongqiang Yang, Gangqiang Ding, Junping Liu, Jia Shang, Yi Kang, Xinying Ji
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?