Rosmarinic acid influences collagen, MMPs, TIMPs, glycosylation and MUC1 in CRL-1739 gastric cancer cell line - 20/09/18
, K. Supruniuk a, J. Nazaruk b, E. Karna c, B. Popławska d, A. Bielawska d, A. Galicka aBienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 11 |
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Graphical abstract |
Highlights |
• | Rosmarinic acid decreases MMP-9 in gastric cancer cells. |
• | RA in gastric cancer inhibits TIMP-1 on protein level and stimulates on mRNA level. |
• | collagen I and COL1A1 mRNA in gastric cancer is stimulated by RA. |
• | Tn, T antigens and their sialylated forms in gastric cancer are inhibited by RA. |
• | RA inhibits protein level of MUC1 mucin in gastric cancer. |
Abstract |
Rosmarinic acid (RA) is a natural phenylpropanoid with numerous pharmacological activities. Because of limited studies of the effects of RA action in gastric cancer cells we examined how 100 and 200 μM acid influences MMPs, TIMPs, collagen, MUC1 and specific sugar antigens in gastric adenocarcinoma CRL-1739 cells. We revealed inhibitory effect of RA on MMP-9 activity what was correlated with increased collagen type I expression, main ECM substrate degraded by MMPs. Tissue inhibitor of MMPs, TIMP-1 but not TIMP-2 was significantly decreased on the protein level and increased on mRNA level by RA action what can suggest TIMP-1 independent inhibitory action of an acid on MMP-9 activity. Glycosylation of gastric cancer proteins was also effected by RA. ELISA tests revealed inhibitory effect of an acid on Tn antigen in cell lysates and culture supernatant and on T antigen in cell lysates. RA inhibited also sialylated Tn antigen in protein of culture supernatant and sialyl T in cell lysates. Extracellular domain of MUC1 mucin, main carrier of Tn and T antigens was significantly inhibited by higher dose of RA. The data suggest potential usefulness of RA as a complementary agent supporting chemotherapy in cancer treatment.
Le texte complet de cet article est disponible en PDF.Keywords : Collagen, Glycosylation, MMPs, MUC1, Rosmarinic acid, TIMPs
Plan
Vol 107
P. 397-407 - novembre 2018 Retour au numéro
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