CXCL9 promotes the progression of diffuse large B-cell lymphoma through up-regulating β-catenin - 20/09/18
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Highlights |
• | CXCL9 is highly expressed in NLBCL tissues and cell lines. |
• | High expression of CXCL9 is positively associated with patients’ advanced clinical progression and shorter overall survival. |
• | Up-regulation of CXCL9 promotes the viability and migration and represses apoptosis of NLBCL cells. |
• | CXCL9 promotes the progression of NLBCL in a β -catenin-dependent manner. |
Abstract |
CXC chemokine ligand 9, a member of “cytokine milieu”, makes up the microenvironment of lymphoma and plays an important role in the occurrence and development of lymphoma. However, the role of CXC chemokine ligand 9 and its underlying mechanism remains largely unknown in the progression of diffuse large B-cell lymphoma. Wnt/ β -catenin signaling is reported to play an important role in diffuse large B-cell lymphoma, and the overexpression and nuclear accentuation of β-catenin in diffuse large B-cell lymphoma patients’ tissues were closely associated with the advanced clinical staging. The present study aimed to explore the effects of CXC chemokine ligand 9 on the progression of diffuse large B-cell lymphoma and determine if β -catenin is involved in this process. We firstly detected the expression pattern of CXC chemokine ligand 9 in diffuse large B-cell lymphoma tissues and cell lines, and determined the relationship between CXC chemokine ligand 9 expression level and patients’ progression and prognosis. Then we used the loss/gain of function approach to determine the effects of CXC chemokine ligand 9 on cell viability, apoptosis and migration. Finally, we assessed the expression and subcellular location of β-catenin after CXC chemokine ligand 9 up/down-regulation by Western Blot. Our results showed that CXC chemokine ligand 9 was highly expressed in diffuse large B-cell lymphoma tissues and cell lines, which showed close association with patients’ advanced clinical progression and shorter overall survival. Up-regulation of CXC chemokine ligand 9 promoted the viability and migration and repressed the apoptosis of OCI-Ly8 cells, as well as increased β-catenin expression and translocated it from cytoplasm to nuclear, while these effects were abolished when knockdown β-catenin. In conclusion, this work reveals that CXC chemokine ligand 9 promotes the progression of diffuse large B-cell lymphoma in a β-catenin-dependent manner. Our study provides evidence for the mechanism by which CXC chemokine ligand 9 may function as an oncogene and the potential of serving CXC chemokine ligand 9 as a therapeutic target for diffuse large B-cell lymphoma.
Le texte complet de cet article est disponible en PDF.Keywords : CXC chemokine ligand 9, β-Catenin, Diffuse large B-cell lymphoma, Prognosis, Progression
Plan
Vol 107
P. 689-695 - novembre 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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