LncRNA HOTAIR suppresses TNF-α induced apoptosis of nucleus pulposus cells by regulating miR-34a/Bcl-2 axis - 20/09/18
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 9 |
| Iconographies | 7 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
HOTAIR inhibits miR-34a expression.
Bcl-2 was a target of miR-34a, and miR-34a was negatively correlated with Bcl-2.
HOTAIR suppresses TNF-α induced apoptosis of NPCs by regulating miR-34a/Bcl-2 axis.
Highlights |
• | HOTAIR expression is down-regulated in nucleus pulposus tissues and cells. |
• | HOTAIR reduced miR-34a induced apoptosis of nucleus pulposus cell. |
• | Bcl-2 was a target of miR-34a and negatively related with miR-34a. |
Abstract |
Objective |
The aim of this study was to investigate the regulation mechanism of HOTAIR on nucleus pulposus cell (NPC) apoptosis induced by inflammatory cytokines.
Methods |
QRT-PCR was performed to analyze the expression of HOTAIR and miR-34a. Safranin O staining and immunohistochemical staining were measured to identify NPCs. ELISA assay was used to detect TNF-α level. Apoptosis was detected with TUNEL assay. Western blotting was measured to analyze the expression of caspase-3, Bax and Bcl-2. TargetScan was used to predict potential targets of miR-34a.
Results |
HOTAIR was significantly low-expressed in degenerative nucleus pulposus (NP) tissues and cells of IDD patients, overexpressing HOTAIR obviously inhibited TNF-α level, NPCs apoptosis and the expression of caspase-3 and Bax, while promoted the expression of Bcl-2. MiR-34a was obviously expressed in degenerative NP tissues and cells of IDD patients. HOTAIR reduced miR-34a induced apoptosis. Apoptotic inhibition gene Bcl-2 is the target gene of miR-34a, and showed a negative relationship with miR-34a.
Conclusion |
Our data suggest that lncRNA HOTAIR suppresses TNF-α induced NPCs apoptosis by regulating miR-34a/Bcl-2 axis in IDD patients.
Le texte complet de cet article est disponible en PDF.Keywords : HOTAIR, Nucleus pulposus cells, miR-34a, Bcl-2
Plan
Vol 107
P. 729-737 - novembre 2018 Retour au numéro
Article précédent
- Protective effects of rutin on liver injury in type 2 diabetic db/db mice
- Weishi Liang, Dandan Zhang, Jiali Kang, Xubing Meng, Jingbo Yang, Lei Yang, Ning Xue, Qingyao Gao, Shuying Han, Xiangbo Gou
- Jinlong Capsule (JLC) inhibits proliferation and induces apoptosis in human gastric cancer cells in vivo and in vitro
- Dan Li, Tengyang Ni, Li Tao, Feng Jin, Haibo Wang, Jun Feng, Guang Zhu, Yayun Qian, Yanbing Ding, Masataka Sunagagwa, Yanqing Liu
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?