Novel folic acid-conjugated doxorubicin loaded β-lactoglobulin nanoparticles induce apoptosis in breast cancer cells - 20/09/18
, Omidreza Firuzi c, ⁎, 1 Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 12 |
| Iconographies | 8 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | Folic acid conjugated β-lactoglobulin nanoparticles (FBNPs) were synthesized for the first time. |
• | Killing breast cancer cells by using doxorubicin (DOX) loaded FBNPs. |
• | Higher cytotoxic effect of DBNPs and FDBNPs in comparison to free DOX. |
Abstract |
Chemotherapy constitutes the main strategy in management of breast cancer (BC). Lack of specificity and high burden of adverse effects of chemotherapeutic agents remain the most important impediments to successful treatment of BC patients. Folate receptor α (FRα) could be very promising for therapeutic targeting in this type of cancer. In this study, ß-lactoglobulin nanoparticles (BNPs) conjugated with folic acid and loaded with doxorubicin (FDBNPs) were prepared. Various characterization techniques were applied to determine the size, polydispersity and doxorubicin loading of prepared FDBNPs in comparison with doxorubicin-loaded BNPs (DBNPs). The results showed that FDBNPs are 109.77 ± 2.80 nm in diameter with well dispersed and spherical shapes. The biodegradation of FDBNPs in the presence of trypsin enzyme and in PBS at different pH (4 and 7) was spectrophotometrically monitored and the results showed that the FDBNPs with encapsulation efficiency of 68.82%±1.76% could deliver doxorubicin at clinically relevant doses. Effects of DBNPs and FDBNPs against MCF-7 and MDA-MB-231, BC and triple negative BC (TNBC) cell lines, respectively, showed significant inhibition of cell proliferation as well as induction of apoptosis. Based on these findings, FDBNPs with facilitated drug release and targeted doxorubicin delivery capacities could have high therapeutic potential for BC and TNBC.
Le texte complet de cet article est disponible en PDF.Abbreviations : BLG, BNPs, DOX, DBNPs, FBNPs, FDBNPs, FR, TNBC, AFM, SEM, DLS, EE, FBS
Keywords : Doxorubicin, ß-Lactoglobulin, Folic acid, Breast cancer
Plan
Vol 107
P. 945-956 - novembre 2018 Retour au numéro
Article précédent
- Apple polyphenol relieves hypoxia-induced pulmonary arterial hypertension via pulmonary endothelium protection and smooth muscle relaxation: In vivo and in vitro studies
- Chunyan Hua, Jie Zhao, Heng Wang, Fangzheng Chen, Hanyan Meng, Luowei Chen, Quanbo Zhang, Junjie Yan, Linbo Yuan
- Garcinol exerts anti-cancer effect in human cervical cancer cells through upregulation of T-cadherin
- Juan Zhao, Ting Yang, Jing Ji, Chen Li, Zhen Li, Long Li
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?