Salidroside promotes angiogenesis after cerebral ischemia in mice through Shh signaling pathway - 27/04/24
, Lina Wang a, b, c, ⁎ Abstract |
Aims |
The purpose of this study was to explore the impacts of salidroside on vascular regeneration, vascular structural changes and long-term neurological recuperation following cerebral ischemia and its possible mechanism.
Main methods |
From Day 1 to Day 28, young male mice with middle cerebral artery blockage received daily doses of salidroside and measured neurological deficits. On the 7th day after stroke, the volume of cerebral infarction was determined using TTC and HE staining. Microvascular density, astrocyte coverage, angiogenesis and the expression of the Shh signaling pathway were detected by IF, qRT-PCR and WB at 7, 14 and 28 days after stroke. Changes in blood flow, blood vessel density and diameter from stroke to 28 days were measured by the LSCI and TPMI.
Key findings |
Compared with the dMACO group, the salidroside treatment group significantly promoted the recovery of neurological function. Salidroside was found to enhance cerebral blood flow perfusion and reduce the infarct on the 7th day after stroke. From the 7th to the 28th day after stroke, salidroside treatment boosted the expression of CD31, CD31+/BrdU+, and GFAP in the cortex around the infarction site. On the 14th day after stroke, salidroside significantly enhanced the width and density of blood vessels. Salidroside increased the expression of histones and genes in the Shh signaling pathway during treatment, and this effect was weakened by the Shh inhibitor Cyclopamine.
Significance |
Salidroside can restore nerve function, improve cerebral blood flow, reduce cerebral infarction volume, increase microvessel density and promote angiogenesis via the Shh signaling pathway.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Salidroside promotes the recovery of neurological function after stroke. |
• | Salidroside enhances cerebral blood flow perfusion and reduces cerebral infarct volume. |
• | Salidroside increases the width and density of blood vessels under two-photon microscope for the first time. |
• | Salidroside is confirmed for the first time to promote stroke recovery and angiogenesis through Shh signaling pathway. |
Abbreviations : Sal, Shh, TTC, IF, WB, LSCI, TPMI, DMCAO, CD31, BrdU, GFAP, Rt-PA, VEGF, Ang1, MNSS, Ptch1, Smo, Gli1, GAPDH
Keywords : Ischemic stroke, Salidroside, Shh signaling pathway, Angiogenesis
Plan
Vol 174
Article 116625- mai 2024 Retour au numéro
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