Limited differentiation to neurons and astroglia from neural stem cells in the cortex and striatum after ischemia/hypoxia in the neonatal rat brain - 18/08/11
, Masanori Iwai, MD b, Takeshi Hayashi, MD b, Isao Nagano, MD b, Mikio Shogi, MD b, Tsuyomu Ikenoue, MD a, Koji Abe, MD b
Reprint requests: Tomoaki Ikeda, MD, Department of Obstetrics and Gynecology, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Kiyotake-cho, Miyazaki, 889-1692 Japan.Abstract |
Objective |
We examined whether progenitor neural stem cells can differentiate successfully into mature neurons and astrocytes in a rat model of neonatal hypoxic-ischemic encephalopathy.
Study design |
Seven-day-old Wistar rats were subjected to hypoxic-ischemic stress. At days 5 to 7 after hypoxic-ischemic stress, 5-bromodeoxyuridine (an early marker of cell proliferation) was injected, and the brains were retrieved at 14, 28, and 42 days after hypoxic-ischemic stress. Immunohistochemical and immunofluorescent studies were carried out for 5-bromodeoxyuridine, neuronal nuclear antigen (a marker protein of matured neuron), and glial fibrillary acidic protein (a protein marker of mature astrocytes).
Results |
Only 1% of neuronal nuclear antigen–positive and 4.6% of glial fibrillary acidic protein–positive cells could be detected among the 5-bromodeoxyuridine-immunopositive cells in the peri-infarcted area of the cortex and the striatum, respectively, at 14 days after hypoxic-ischemic stress. There were no such double-staining cells at 28 and 42 days after hypoxic-ischemic stress.
Conclusion |
The intrinsic ability for neurologic self-repair was limited at the maturation step after hypoxic-ischemic stress in the neonatal rat brain.
Le texte complet de cet article est disponible en PDF.Key words : Hypoxic-ischemic encephalopathy, Neonatal rat, Neural stem cell, Differentiation
Plan
| Supported in part by Grants-in-Aid for Scientific Research (B) 15390273 and (Hoga) 15659338 and the National Project on Protein Structural and Functional Analyses from the Ministry of Education, Science, Culture and Sports of Japan and by grants from the Ministry of Health and Welfare of Japan. |
Vol 193 - N° 3
P. 849-856 - septembre 2005 Retour au numéro
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