Paclitaxel loaded poly-d,l-lactide-co-glycolide nanoparticles: Enhanced anticancer effect in non-small cell lung carcinoma cell line - 01/03/13
, B. Venkadesh b, R. Hussain b, N. Rajendra Prasad c, S.M.A. Shibli d, Anupama V. Raj d, Sheeja S. Rajan eAbstract |
The aim of the present study was to develop PTX-PLGA nanoparticles and to study its anticancer efficacy in NCI-H460 cells in vitro. DLS results revealed that prepared PTX-PLGA had an average size of 211nm. TEM analysis showed that prepared PTX-PLGA nanoparticles were spherical in shape with 150nm. Drug encapsulation assay showed that 65% PTX was encapsulated in PLGA. It was noticed that anticancer potential of PTX-PLGA nanoparticles was greater than that of PTX treatment alone. Further, PLGA-PTX exhibits increased intracellular ROS levels, altered MMP, increased DNA damage, enhanced TBARS and apoptotic morphological changes in NCI-H460 cells when compared to bulk PTX treatment alone. Our results indicate that PTX-PLGA demonstrated increased anticancer property in cancer cells than PTX treatment alone.
Le texte complet de cet article est disponible en PDF.Keywords : Nanoparticles, Anticancer, Paclitaxel (PTX), Poly-d,l-lactide-co-glycolide (PLGA)
Plan
Vol 3 - N° 1
P. 1-9 - janvier 2013 Retour au numéro
- Cytotoxic and antiproliferative effects of crude amphibian skin secretions on breast tumor cells
- Juliana Mozer Sciani, Paulo Luiz de-Sá-Júnior, Adilson Kleber Ferreira, Alexandre Pereira, Marta M. Antoniazzi, Carlos Jared, Daniel Carvalho Pimenta
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?