Antioxidant activity and anticancer effect of bioactive peptide from enzymatic hydrolysate of oyster (Saccostrea cucullata) - 13/08/14

Doi : 10.1016/j.bionut.2014.04.006

S. Umayaparvathi ^a,^⁎ , S. Meenakshi ^a, V. Vimalraj ^b, M. Arumugam ^a, G. Sivagami ^c, T. Balasubramanian ^a
^a Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India
^b Department of Chemistry, Annamalai University, Annamalai Nagar 608002, Chidambaram, Tamil Nadu, India
^c Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India

^⁎Corresponding author. Tel.: +04144 230229.

Abstract

The antioxidant and anticancer activities of bioactive peptide isolated from oyster (Saccostrea cucullata) protein hydrolysate were evaluated in vitro. The oyster hydrolysate exhibited a strong antioxidant potential as a DPPH scavenger (85.7±0.37%) followed by reducing power (2.63±0.2 OD at 700nm) at a concentration of 1mg/ml. Due to the high antioxidant potential, hydrolysate was fractionated in Sephadex G-25 gel filtration chromatography and peptides were purified by UPLC-MS. Among 7 purified peptides (SCAP1–7), 3 peptides (SCAP1, 3 and 7) had the highest scavenging ability on DPPH radicals. The amino acid sequence and molecular mass of purified peptides (SCAP1, SCAP3 and SCAP7) were Leu-Ala-Asn-Ala-Lys (MW=515.29Da), Pro-Ser-Leu-Val-Gly-Arg-Pro-Pro-Val-Gly-Lys-Leu-Thr-Leu (MW=1432.89Da) and Val-Lys-Val-Leu-Leu-Glu-His-Pro-Val-Leu (MW=1145.75Da), respectively. Moreover, oyster peptide SCAP1 had anticancer activity against human colon carcinoma (HT-29) cell lines. Percentage of cell growth inhibition (MTT assay), apoptotic morphological changes (AO/EtBr staining) and oxidative DNA damage (comet assay) were estimated. We thus conclude that the anticancer and antioxidative peptide (SCAP1) from oyster (S. cucullata) may be useful ingredients in pharmaceutical and nutraceutical applications.