The preterm placental microbiome varies in association with excess maternal gestational weight gain - 27/04/15
Abstract |
Objective |
Although a higher maternal body mass index is associated with preterm birth, it is unclear whether excess gestational weight gain (GWG) or obesity drives increased risk. We and others have shown that the placenta harbors microbiota, which is significantly different among preterm births. Our aim in this study was to investigate whether the preterm placental microbiome varies by virtue of obesity or alternately by excess GWG.
Study Design |
Placentas (n = 320) were collected from term and preterm pregnancies. Genomic DNA was extracted and subjected to metagenomic sequencing. Data were analyzed by clinical covariates that included the 2009 Institute of Medicine’s GWG guideline and obesity.
Results |
Analysis of 16S recombinant RNA–based metagenomics revealed no clustering of the microbiome by virtue of obesity (P = .161). Among women who spontaneously delivered preterm, there was again no clustering by obesity (P = .480), but there was significant clustering by excess GWG (P = .022). Moreover, among preterm births, detailed analysis identified microbial genera (family and genus level) and bacterial metabolic gene pathways that varied among pregnancies with excess GWG. Notably, excess GWG was associated with decreased microbial folate biosynthesis pathways and decreased butanoate metabolism (linear discriminate analysis, >3.0-fold).
Conclusion |
Although there were no significant alterations in the microbiome by virtue of obesity per se, excess GWG was associated with an altered microbiome and its metabolic profile among those women who experienced a preterm birth.
Le texte complet de cet article est disponible en PDF.Key words : excess gestational weight gain, maternal obesity, metagenomics, microbiome, preterm birth
Plan
Supported by National Institutes of Health Director’s New Innovator Award number DP2 DP21DP2OD001500, Burroughs Welcome Fund PTB Initiative grant number 1008819.01, and National Institute of Nursing Research Grant number R01NR014792 (all to K.A.) and the Human Microbiome Project funded through the National Institutes of Health Director’s Common Fund. All sequencing and adaptation of protocols for sequencing low-biomass samples were performed by the Baylor College of Medicine Human Genome Sequencing Center, which is funded by direct support from the National Human Genome Research Institute, National Institutes of Health, under grant number U54HG004973. |
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The authors report no conflict of interest. |
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Cite this article as: Antony KM, Ma J, Mitchell KB, et al. The preterm placental microbiome varies in association with excess maternal gestational weight gain. Am J Obstet Gynecol 2015;212:653.e1-16. |
Vol 212 - N° 5
P. 653.e1-653.e16 - mai 2015 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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