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Comptes Rendus Biologies
Volume 333, n° 5
pages 395-404 (mai 2010)
Doi : 10.1016/j.crvi.2010.02.002
Received : 11 January 2010 ;  accepted : 1 February 2010
Biologie et génétique moléculaires / Molecular bilogy and genetics
Genetic identification of Southern Ocean octopod samples using mtCOI
 

Eivind Andreas Baste Undheim a, b, Janette Ann Norman b, Hanne Halkinrud Thoen a, b, Bryan Grieg Fry b, ⁎
a Norwegian University of Technology and Science, Department of Biology, Trondheim Biological Station, N-7491, Trondheim, Norway 
b Department of Biochemistry & Molecular Biology, Bio21 Institute, University of Melbourne, Melbourne, Victoria 3010, Australia 

Corresponding author.
Abstract

East Antarctic octopods were identified by sequencing mtCOI and using four analytical approaches: Neighbor-joining by Kimura-2-Parameter-based distances, character-based, BLAST, and Bayesian Inference of Phylogeny. Although the distance-based analytical approaches identified a high proportion of the sequences (99.5% to genus and 88.1% to species level), these results are undermined by the absence of a clear gap between intra- and interspecific variation. The character-based approach gave highly conflicting results compared to the distance-based methods and failed to identify apomorphic characters for many of the species. While a DNA independent approach is necessary for validation of the method comparisons, crude morphological observations give early support to the distance-based results and indicate extensive range expansions of several species compared to previous studies. Furthermore, the use of distance-based phylogenetic methods nevertheless group specimens into plausible species clades that are highly useful in non-taxonomical or non-systematic studies.


Keywords : Octopus, mtCOI, Pareledone, Adelieledone, Southern Ocean, Antarctica


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