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Comptes Rendus Chimie
Volume 14, n° 9
pages 811-818 (septembre 2011)
Doi : 10.1016/j.crci.2011.02.002
Received : 30 November 2010 ;  accepted : 1 February 2011
Challenges in the directed evolution of stereoselective enzymes for use in organic chemistry
 

M.T. Reetz , G.P.L. Krebs
Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, Germany 

Corresponding author.
Abstract

Directed evolution constitutes an ideal method for engineering essentially any catalytic parameter of enzymes for application in synthetic organic chemistry and biotechnology, including thermostability, substrate scope and enantioselectivity. Stereoselectivity is especially important when applying biocatalysts to synthetic organic chemistry. This article focuses on recent methodology developments in laboratory evolution of stereoselective enzymes, hydrolases and monooxygenases serving as the enzymes. Iterative saturation mutagenesis (ISM) has been developed as an unusually effective method to evolve enhanced or reversed enantioselectivity, broader substrate scope and/or higher thermostability of enzymes.

The full text of this article is available in PDF format.
Résumé

L’évolution dirigée constitue une méthode idéale pour modifier essentiellement n’importe quel paramètre des enzymes ayant une application en synthèse organique et en biotechnologie ; cela inclue la thermostabilité, l’adaptabilité au substrat et l’énantioselectivité. La stéréoselectivité est particulièrement importante lorsque des biocatalyseurs sont utilisés en synthèse organique. Cet article se concentre sur les récents développements en méthodologie de l’évolution au laboratoire des enzymes stéréoselectives ; hydrolases et monooxygenases étant les enzymes utilisées. La mutagénése par saturation itérative (ISM) a été développée comme une méthode exceptionnellement efficace pour obtenir une meilleure énantiosélectivité, ou bien une énantioselectivité inverse, une plus grande adaptabilité au substrat et/ou une plus grande thermostabilité des enzymes.

The full text of this article is available in PDF format.

Keywords : Asymmetric, Catalysis, Biocatalysis, Directed evolution, Enantioselectivity, Enzymes

Mots clés : Catalyse asymétrique, Biocatalyse, Évolution dirigée, Énantioselectivité, Enzyme




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