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Biomedicine and Preventive Nutrition
Volume 2, n° 4
pages 287-293 (octobre 2012)
Doi : 10.1016/j.bionut.2012.03.002
Received : 6 February 2012 ;  accepted : 30 Mars 2012
Original articles

Luffa acutangula and Lippia nodiflora leaf extract induces growth inhibitory effect through induction of apoptosis on human lung cancer cell line
 

R. Vanajothi a, A. Sudha a, R. Manikandan b, P. Rameshthangam c, P. Srinivasan a,
a Department of Bioinformatics, Alagappa University, Alagappapuram, Karaikudi, 630004 Tamilnadu, India 
b Department of Animal Health and Management, Alagappa University, Alagappapuram, Karaikudi, 630004 Tamilnadu, India 
c Department of Biotechnology (DDE), Alagappa University, Alagappapuram, Karaikudi, 630004 Tamilnadu, India 

Corresponding author. Tel.: +91 9444482814; fax: +91 4565 225202.
Graphical abstract

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Abstract

Luffa acutangula (Cucurbitaceae) and Lippia nodiflora (Verbenaceae) have been reputed in the indigenous medicine and claimed to possess a wide array of biological activities including anticancer activity. The present study was designed to assess the in vitro anticancer effect of L.  acutangula and L.  nodiflora leaf extracts in human lung cancer cell line (NCI-H460). The anticancer activity of both extracts was compared with that of paclitaxel as positive control and the effect was assessed by MTT assay, mitochondrial membrane potential (MMP) alteration, intracellular reactive oxygen species (ROS) measurement and nuclear morphology with the appearance of apoptosis. The leaf extracts exhibits high antiproliferative activity against cell line tested, as determined with MTT assay. The concentrations of growth inhibition at 50% (IC50 ) ranged from 1 to 50μg/mL after 24h treatment and the IC50 was found to be 20μg/mL and 10μg/mL for L.  acutangula and L.  nodiflora , respectively. Both extract treated groups exhibits high DCF fluorescence (enhanced ROS levels), significant increase in mitochondrial depolarization when compared to control groups. Nuclear morphology with induction of apoptosis in cells treated with leaf extracts were also observed by microscopic examination using dual staining method of acridine orange-ethidium bromide. The present study suggests the further research such as fractionation and purification of bioactive compounds in order to formulate natural compounds with anticancer activities.

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Keywords : Luffa acutangula , Lippia nodiflora , NCI-H460 cell lines, ROS, Antiproliferative activity, Apoptosis




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