S'abonner

Nasal IL-4+CXCR5+CD4+ T follicular helper cell counts correlate with local IgE production in eosinophilic nasal polyps - 05/02/16

Doi : 10.1016/j.jaci.2015.07.025 
Ya-Na Zhang, MD, PhD a, b, Jia Song, MD a, Hai Wang, MD a, Heng Wang, MD, PhD a, Ming Zeng, MD, PhD a, Guan-Ting Zhai, MD a, Jin Ma, MD a, Zhi-Yong Li, MD a, Bo Liao, MD a, Bao-Feng Wang, MD a, Zhen Zhen, MD, PhD a, d, Nan Wang, MD, PhD a, Ping-Ping Cao, MD, PhD a, Peng Lin, MD b, Qin Ning, MD, PhD c, Zheng Liu, MD, PhD a,
a Department of Otolaryngology–Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China 
c Department of Infectious Disease, Institute of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China 
b Department of Otolaryngology–Head and Neck Surgery, Tianjin First Center Hospital, Tianjin, China 
d Department of Otolaryngology–Head and Neck Surgery, Peking University First Hospital, Beijing, China 

Corresponding author: Zheng Liu, MD, PhD, Department of Otolaryngology–Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jiefang Ave, Wuhan 430030, P.R. China.

Abstract

Background

Locally produced IgE contributes to the initiation and development of eosinophilic inflammation in eosinophilic nasal polyps independent of systemic atopy. However, whether CXCR5+CD4+ T follicular helper (TFH) cells are involved in local IgE production at mucosal sites remains unexplored.

Objective

We sought to explore the presence, phenotype, and function of CXCR5+CD4+ TFH cells in eosinophilic nasal polyp tissues compared with noneosinophilic nasal polyp and control normal nasal tissues.

Methods

TFH cell-surface phenotypes and subsets and B-cell subsets in nasal tissues and peripheral blood were studied by means of flow cytometry. Immunohistochemistry was used to detect the tissue location of TFH cells. Sorted nasal TFH cells and CXCR5 T cells were cultured with autologous naive B cells purified from blood.

Results

Nasal TFH cells expressed inducible costimulator, programmed cell death protein 1, and the transcription factor B-cell lymphoma 6 (Bcl-6) at an intermediate level when compared with bona fide TFH cells in tonsils and circulating TFH cells. Although counts of total TFH cells and IL-21+, IFN-γ+, and IL-17+ TFH cells were increased in both eosinophilic and noneosinophilic nasal polyp tissues compared with those in normal nasal tissues, IL-4+ TFH cell counts were only increased in eosinophilic polyp tissues. IL-4 and IL-21 were involved in polyp TFH cell–induced IgE production from naive B cells, and nasal IL-4+ TFH cell counts correlated highly with local IgE levels in vivo. IL-4+Bcl-6+CD4+ TFH cells were identified in ectopic lymphoid structures in eosinophilic nasal polyps. TFH cells also positively correlated with germinal center B cells and plasma cells in nasal tissues.

Conclusion

Nasal IL-4+ TFH cells might be involved in local IgE production in eosinophilic nasal polyps.

Le texte complet de cet article est disponible en PDF.

Key words : B cell, ectopic lymphoid structure, eosinophil, IgE, IL-4, nasal polyp, T follicular helper cell

Abbreviations used : Bcl-6, Bm, CRSwNP, CSR, FACS, Foxp3, GC, ICOS, NMC, PD1, TFH


Plan


 Supported by National Natural Science Foundation of China (NSFC) grants 81020108018 and 81325006 (to Z.L.), 81200733 (to H.W.), and 81400449 (to P.-P.C.); a grant from the Ministry of Health of China (201202005); the Program for Changjiang Scholars and Innovative Research Team in University (IRT_14R20); and the 12th 5-year science and technology support program (2014BAI07B04).
 Disclosure of potential conflict of interest: H. Wang has received a grant from the National Natural Science Foundation of China (81200733). P.-P. Cao has received a grant from the National Natural Science Foundation of China (81400449). Q. Ning has received a grant from the Ministry of Education of China (Program for Changjiang Scholars and Innovative Research Team in University [IRT_14R20]). Z. Liu has received grants from the National Natural Science Foundation of China (81020108018 and 81325006), the Ministry of Science and Technology of China (2014BAI07B04), and the Ministry of Health of China (201202005). The rest of the authors declare that they have no relevant conflicts of interest.


© 2015  American Academy of Allergy, Asthma & Immunology. Publié par Elsevier Masson SAS. Tous droits réservés.
Ajouter à ma bibliothèque Retirer de ma bibliothèque Imprimer
Export

    Export citations

  • Fichier

  • Contenu

Vol 137 - N° 2

P. 462-473 - février 2016 Retour au numéro
Article précédent Article précédent
  • Short-term subcutaneous grass pollen immunotherapy under the umbrella of anti–IL-4: A randomized controlled trial
  • Adam M. Chaker, Mohamed H. Shamji, Florentina A. Dumitru, Moises A. Calderon, Guy W. Scadding, Melina Makatsori, Ieuan Jones, Qiuling A. He, Kulandayan K. Subramanian, Jonathan P. Arm, Stephen R. Durham, Carsten B. Schmidt-Weber
| Article suivant Article suivant
  • Timing and duration of omalizumab response in patients with chronic idiopathic/spontaneous urticaria
  • Allen Kaplan, Marta Ferrer, Jonathan A. Bernstein, Evgeniya Antonova, Benjamin Trzaskoma, Karina Raimundo, Karin Rosén, Theodore A. Omachi, Sam Khalil, James L. Zazzali

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

Déjà abonné à cette revue ?

Mon compte


Plateformes Elsevier Masson

Déclaration CNIL

EM-CONSULTE.COM est déclaré à la CNIL, déclaration n° 1286925.

En application de la loi nº78-17 du 6 janvier 1978 relative à l'informatique, aux fichiers et aux libertés, vous disposez des droits d'opposition (art.26 de la loi), d'accès (art.34 à 38 de la loi), et de rectification (art.36 de la loi) des données vous concernant. Ainsi, vous pouvez exiger que soient rectifiées, complétées, clarifiées, mises à jour ou effacées les informations vous concernant qui sont inexactes, incomplètes, équivoques, périmées ou dont la collecte ou l'utilisation ou la conservation est interdite.
Les informations personnelles concernant les visiteurs de notre site, y compris leur identité, sont confidentielles.
Le responsable du site s'engage sur l'honneur à respecter les conditions légales de confidentialité applicables en France et à ne pas divulguer ces informations à des tiers.


Tout le contenu de ce site: Copyright © 2024 Elsevier, ses concédants de licence et ses contributeurs. Tout les droits sont réservés, y compris ceux relatifs à l'exploration de textes et de données, a la formation en IA et aux technologies similaires. Pour tout contenu en libre accès, les conditions de licence Creative Commons s'appliquent.