Nasal IL-4+CXCR5+CD4+ T follicular helper cell counts correlate with local IgE production in eosinophilic nasal polyps - 05/02/16
Abstract |
Background |
Locally produced IgE contributes to the initiation and development of eosinophilic inflammation in eosinophilic nasal polyps independent of systemic atopy. However, whether CXCR5+CD4+ T follicular helper (TFH) cells are involved in local IgE production at mucosal sites remains unexplored.
Objective |
We sought to explore the presence, phenotype, and function of CXCR5+CD4+ TFH cells in eosinophilic nasal polyp tissues compared with noneosinophilic nasal polyp and control normal nasal tissues.
Methods |
TFH cell-surface phenotypes and subsets and B-cell subsets in nasal tissues and peripheral blood were studied by means of flow cytometry. Immunohistochemistry was used to detect the tissue location of TFH cells. Sorted nasal TFH cells and CXCR5− T cells were cultured with autologous naive B cells purified from blood.
Results |
Nasal TFH cells expressed inducible costimulator, programmed cell death protein 1, and the transcription factor B-cell lymphoma 6 (Bcl-6) at an intermediate level when compared with bona fide TFH cells in tonsils and circulating TFH cells. Although counts of total TFH cells and IL-21+, IFN-γ+, and IL-17+ TFH cells were increased in both eosinophilic and noneosinophilic nasal polyp tissues compared with those in normal nasal tissues, IL-4+ TFH cell counts were only increased in eosinophilic polyp tissues. IL-4 and IL-21 were involved in polyp TFH cell–induced IgE production from naive B cells, and nasal IL-4+ TFH cell counts correlated highly with local IgE levels in vivo. IL-4+Bcl-6+CD4+ TFH cells were identified in ectopic lymphoid structures in eosinophilic nasal polyps. TFH cells also positively correlated with germinal center B cells and plasma cells in nasal tissues.
Conclusion |
Nasal IL-4+ TFH cells might be involved in local IgE production in eosinophilic nasal polyps.
Le texte complet de cet article est disponible en PDF.Key words : B cell, ectopic lymphoid structure, eosinophil, IgE, IL-4, nasal polyp, T follicular helper cell
Abbreviations used : Bcl-6, Bm, CRSwNP, CSR, FACS, Foxp3, GC, ICOS, NMC, PD1, TFH
Plan
Supported by National Natural Science Foundation of China (NSFC) grants 81020108018 and 81325006 (to Z.L.), 81200733 (to H.W.), and 81400449 (to P.-P.C.); a grant from the Ministry of Health of China (201202005); the Program for Changjiang Scholars and Innovative Research Team in University (IRT_14R20); and the 12th 5-year science and technology support program (2014BAI07B04). |
|
Disclosure of potential conflict of interest: H. Wang has received a grant from the National Natural Science Foundation of China (81200733). P.-P. Cao has received a grant from the National Natural Science Foundation of China (81400449). Q. Ning has received a grant from the Ministry of Education of China (Program for Changjiang Scholars and Innovative Research Team in University [IRT_14R20]). Z. Liu has received grants from the National Natural Science Foundation of China (81020108018 and 81325006), the Ministry of Science and Technology of China (2014BAI07B04), and the Ministry of Health of China (201202005). The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 137 - N° 2
P. 462-473 - février 2016 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?