The incidence of food allergies has increased dramatically during the last decade. Recently, probiotics have been studied for the prevention and treatment of allergic disease.
We examined whether Bifidobacterium longum KACC 91563 and Enterococcus faecalis KACC 91532 have the capacity to suppress food allergies.
B longum KACC 91563 and E faecalis KACC 91532 were administered to BALB/c wild-type mice, in which food allergy was induced by using ovalbumin and alum. Food allergy symptoms and various immune responses were assessed.
B longum KACC 91563, but not E faecalis KACC 91532, alleviated food allergy symptoms. Extracellular vesicles of B longum KACC 91563 bound specifically to mast cells and induced apoptosis without affecting T-cell immune responses. Furthermore, injection of family 5 extracellular solute-binding protein, a main component of extracellular vesicles, into mice markedly reduced the occurrence of diarrhea in a mouse food allergy model.
B longum KACC 91563 induces apoptosis of mast cells specifically and alleviates food allergy symptoms. Accordingly, B longum KACC 91563 and family 5 extracellular solute-binding protein exhibit potential as therapeutic approaches for food allergies.Le texte complet de cet article est disponible en PDF.
Key words : Food allergy, Bifidobacterium longum, probiotics, extracellular vesicle, mast cells, family 5 extracellular solute-binding protein, ovalbumin
Abbreviations used : ASBP, BMMC, cfu, DC, DC-SIGN, ESBP, EV, FISH, Foxp3, GST, LP, MCPT-1, MHCII, MLN, OVA, TCR, TEM, Treg, TUNEL
| Supported by the Institute for Basic Science (IBS; IBS-R005-S1-2015-a00 and IBS-R005-D1-2015-a00), the National Institute of Animal Science research project of Rural Development Administration of Korea (PJ00932901), and the National Research Foundation of Korea (NRF; 2013-056085).
| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest.