Comparison of 3 type VII collagen (C7) assays for serologic diagnosis of epidermolysis bullosa acquisita (EBA) - 15/05/16
Abstract |
Background |
Serologic diagnosis of epidermolysis bullosa acquisita (EBA) relies on the detection of circulating autoantibodies to type VII collagen (C7).
Objective |
We sought to compare the diagnostic performances of a commercialized enzyme-linked immunosorbent assay (ELISA) using C7 noncollagenous (NC) domains (C7-NC1/NC2 ELISA) and indirect immunofluorescence (IIF) biochip test on NC1-C7-expressing transfected cells (IIFT), with a full-length–C7 ELISA developed in our laboratory.
Methods |
C7-NC1/NC2 ELISA, IIFT, and full-length–C7 ELISA were run on 77 nonselected consecutive EBA sera.
Results |
C7-NC1/NC2 ELISA, IIFT, and full-length–C7 ELISA were positive, respectively, for: 30%, 27%, and 65% of the 77 sera; 43%, 32%, and 80% of 44 sera labeling the salt-split-skin (SSS) floor (F) by IIF (SSS/F+); 9%, 22%, and 47% of 32 SSS/F− sera; 28%, 28%, and 58% of classic EBA; 41%, 41%, and 82% of inflammatory EBA; and 18%, 0%, and 55% of mucous-membrane-predominant EBA. Significant differences for all sera were found between: the 2 ELISAs for the 77 sera, SSS/F+ and SSS/F− sera, and IIFT versus full-length–C7 ELISA.
Limitations |
The retrospective design was a limitation.
Conclusion |
C7-NC1/NC2 ELISA and IIFT sensitivities for serologic diagnoses of EBA were low. Full-length–C7 ELISA was significantly more sensitive and could serve as a reference test.
Le texte complet de cet article est disponible en PDF.Key words : autoimmune bullous disease, biochip, enzyme-linked immunosorbent assay, epidermolysis bullosa acquisita, indirect immunofluorescence, sensitivity, serologic diagnosis, type VII collagen
Abbreviations used : AFz, AIBD, AU, BP, C7, cEBA, EBA, ELISA, iEBA, IEM, IIF, IIFT, MMp, NC, NHS, SSS, SSS/F+, SSS/F−
Plan
| Drs Prost-Squarcioni and Hovnanian have equally contributed to this work and share last authorship. |
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| Supported by GENEGRAFT FP17 European Project and Dystrophic Epidermolysis Bullosa Research Association–France. |
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| Conflicts of interest: None declared. |
Vol 74 - N° 6
P. 1166-1172 - juin 2016 Retour au numéro
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