Indoor microbial communities: Influence on asthma severity in atopic and nonatopic children - 01/07/16
Abstract |
Background |
Allergic and nonallergic asthma severity in children can be affected by microbial exposures.
Objective |
We sought to examine associations between exposures to household microbes and childhood asthma severity stratified by atopic status.
Methods |
Participants (n = 196) were selected from a cohort of asthmatic children in Connecticut and Massachusetts. Children were grouped according to asthma severity (mild with no or minimal symptoms and medication or moderate to severe persistent) and atopic status (determined by serum IgE levels). Microbial community structure and concentrations in house dust were determined by using next-generation DNA sequencing and quantitative PCR. Logistic regression was used to explore associations between asthma severity and exposure metrics, including richness, taxa identification and quantification, community composition, and concentration of total fungi and bacteria.
Results |
Among all children, increased asthma severity was significantly associated with an increased concentration of summed allergenic fungal species, high total fungal concentrations, and high bacterial richness by using logistic regression in addition to microbial community composition by using the distance comparison t test. Asthma severity in atopic children was associated with fungal community composition (P = .001). By using logistic regression, asthma severity in nonatopic children was associated with total fungal concentration (odds ratio, 2.40; 95% CI, 1.06-5.44). The fungal genus Volutella was associated with increased asthma severity in atopic children (P = .0001, q = 0.04). The yeast genera Kondoa might be protective; Cryptococcus species might also affect asthma severity.
Conclusion |
Asthma severity among this cohort of children was associated with microbial exposure, and associations differed based on atopic status.
Le texte complet de cet article est disponible en PDF.Key words : Allergy, asthma severity, atopy, bacteria, children, DNA sequencing, fungi, indoor environment, microbial communities, nonallergic asthma
Abbreviations used : ITS, OTU, qPCR
Plan
| Supported by the Alfred P. Sloan Foundation (Microbiology of the Build Environment) and a grant from the National Institutes of Health (ES05410). |
|
| Disclosure of potential conflict of interest: B. P. Leaderer receives research support from National Institute of Environmental Health Sciences ES05410. J. Peccia receives research support from the Alfred P. Sloan Foundation. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 138 - N° 1
P. 76 - juillet 2016 Retour au numéro
Article précédent
- Multidimensional endotyping in patients with severe asthma reveals inflammatory heterogeneity in matrix metalloproteinases and chitinase 3–like protein 1
- Timothy S.C. Hinks, Tom Brown, Laurie C.K. Lau, Hitasha Rupani, Clair Barber, Scott Elliott, Jon A. Ward, Junya Ono, Shoichiro Ohta, Kenji Izuhara, Ratko Djukanovi?, Ramesh J. Kurukulaaratchy, Anoop Chauhan, Peter H. Howarth
- House dust mite–induced asthma causes oxidative damage and DNA double-strand breaks in the lungs
- Tze Khee Chan, Xin Yi Loh, Hong Yong Peh, W.N. Felicia Tan, W.S. Daniel Tan, Na Li, Ian J.J. Tay, W.S. Fred Wong, Bevin P. Engelward
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?