Oncogenic long noncoding RNA MALAT1 and HCV-related hepatocellular carcinoma - 30/04/18
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Highlights |
• | Higher MALAT1 serum levels were observed in HCC vs. cirrhotic patients and controls. |
• | Higher MALAT1 level was associated with male gender, massive ascites, and AFP level. |
• | Serum MALAT1 profile was positively correlated with liver cell failure scores. |
• | Meta-analysis results confirmed MALAT1 oncogenic role in HCV-induced HCC. |
Abstract |
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related mortality worldwide. The oncogenic function of the long non-coding RNA; metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in HCC remains unclear. We aimed to evaluate MALAT1 serum expression profile in HCC and explore its relation to the clinicopathological features. Quantitative Real Time-Polymerase Chain Reaction was applied in 70 cohorts (30 HCC, 20 HCV, 20 controls). Further meta-analysis of clinical studies and in vitro validated experiments was employed. Serum MALAT1 showed area under the curve of 0.79 and 0.70 to distinguish patients with cancer from normal and cirrhotic individuals at fold change of 1.0 and 1.26, respectively. Expression level was significantly higher in males (P <0.001) and patients with massive ascites (P = 0.005). Correlation analysis showed positive correlation of MALAT1 with total bilirubin (r = 0.456, P <0.001) and AST (r = 0.280, P = 0.019), and negative correlation with the hemoglobin level (r = 0.312, P = 0.009). Meta-analysis showed that the over-expressed MALAT1 was linked to tumor number [Cohen's d = 0.450, 95% CI (0.21 to 0.68)], clinical stage [Cohen's d = 0.048, 95% CI (-0.83 to 0.74)], and AFP level [Cohen's d = 0.354, 95% CI (0.1 to 0.57)]. In silico data analysis and systematic review confirmed MALAT1 oncogenic function in cancer development and progression.
In conclusion, circulatory MALAT1 might represent a putative non-invasive prognostic biomarker indicating worse liver failure score in HCV-related HCC patients with traditional markers. Large-scale verification is warranted in future studies.
Le texte complet de cet article est disponible en PDF.Abbreviations : AFP, AUC, cDNA, DRAIC, EMT, eRNAs, EZH2, GAS5, HCC, HCN, HCV, HEIH, HULC, ITGB1, LINC00047, lncRNAs, LTBP3, MALAT1, mascRNA, ncRNA-a, NEAT2, OncoLncs, plncRNAs, PRC, ROC, RT-PCR, siRNA, TF, TS, TSLncs
Keywords : MALAT1, HCV, HCC, lncRNA, Meta-analysis
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Vol 102
P. 653-669 - juin 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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