Tetramethylpyrazine (TMP) ameliorates corneal neovascularization via regulating cell infiltration into cornea after alkali burn - 09/12/18
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 11 |
| Iconographies | 8 |
| Vidéos | 0 |
| Autres | 0 |
Highlights |
• | Alkali burn induces upregulation of CXCR4 in cornea. |
• | TMP restrains CXCR4 expression and vasculogenesis both in vitro and in vivo. |
• | TMP and FK506 restrain inflammatory cells infiltration in alkali burn cornea. |
• | TMP and FK506 regulate different cytokines infiltration in alkali burn cornea. |
• | TMP and FK506 are equally effective in inhibiting CNV after alkali burn. |
Abstract |
In the present study, we investigated the underlying mechanism of tetramethylpyrazine (TMP)-medicated inhibition of corneal neovascularization (CNV). Our data showed that TMP could effectively downregulate the expression levels of CXCR4 mRNA and protein, as well as inhibit HUVECs, endothelial cells, tubule formation in vitro. In vivo, alkali burn (1 M NaOH) could remarkably upregulate CXCR4 expression and increase the migration of TNF-α-positive cells to corneal stroma. TMP drops could significantly downregulate CXCR4 expression in cornea, compared to the control. However, there was no difference in the downregulation of CXCR4 between TMP and FK506, an immunosuppressive drug. Moreover, the immunofluorescent staining of CD45 showed TMP and FK506 could significantly restrain the bone marrow (BM)-derived infiltration while the F4/80 staining reflects the suppression of macrophage aggregation. Meanwhile TMP could regulate the Interleukin 10 (IL-10) and FK506 could restrain the Interleukin 2 (IL-2). Furthermore, TMP and FK506 significantly ameliorate corneal opacity and neovascularization. Clinical assessment detected an obvious improvement in TMP and FK506 treatment groups, compared to controls in vivo. Thus, TMP had similar effects in inhibition of immune response and CNV by suppressing BM-infiltrating cells into cornea as FK506. TMP could be a potential agent in eye-drop therapy for cornea damaged by Alkali Burn.
Le texte complet de cet article est disponible en PDF.Abbreviations : CNV, NS, FK506, TMP, CXCR4, IL-2, IL-10
Keywords : Alkali burn, Cell infiltration, Corneal neovascularization (CNV), CXC chemokine receptor type 4 (CXCR4), Tetramethylpyrazine (TMP)
Plan
Vol 109
P. 1041-1051 - janvier 2019 Retour au numéro
Article précédent
- RETRACTED: Neferine inhibits proliferation, migration and invasion of U251 glioma cells by down-regulation of miR-10b
- Hua-Xin Liang, Li-Bo Sun, Nai-Jie Liu
- Protective and reversal actions of a novel peptidomimetic against a pivotal toxin implicated in Alzheimer’s disease
- Giovanni Ferrati, Georgi Bion, Andrew J. Harris, Susan Greenfield
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?