Zhen-wu-tang attenuates Adriamycin-induced nephropathy via regulating AQP2 and miR-92b - 09/12/18
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Graphical abstract |
Highlights |
• | Aquaorin-2 and microRNAs are critical in nephrotic syndrome (NS) pathogenesis. |
• | miR-92b regulated AQP2 expression by binding to the 3′-UTR of AQP2 transcript. |
• | ZWT ameliorated NS edema and tubular injury by targeting AQP2 and miR-92b. |
• | Advanced understanding of NS pathogenesis and pointed a new therapeutic method. |
Abstract |
Nephrotic syndrome (NS) is characterized by proteinuria, hypoalbuminemia and edema. The disorder of sodium and water metabolism is a critical mechanism regulating the origination and progression of NS. Zhen-wu-tang (ZWT) has been traditionally used to treat edema disease in China and Japan. The present study was carried out to assess the protective effect of ZWT in Adriamycin-induced (ADR) NS rats and investigate the potential anti-NS mechanisms of ZWT. We found that ZWT treatment ameliorate impaired kidney function and regulate water balance of kidney. Importantly, ZWT increased the expression of Aquaporin-2 (AQP2) which play key roles in maintaining body water homeostasis. Additionally, we determined miRNAs expression patterns in NS rats. Using bioinformatics prediction and miR-92b mimic or inhibitor in vitro, we identified miR-92b as a possible modulator of AQP2. Also we found that ZWT can decrease the expression of miR-92b and reverse the effect of miR-92b on AQP2 in vitro. We further demonstrated that miR-92b directly regulated AQP2 expression by targeting 3′-UTR of AQP2. These finding suggest that ZWT may reduce renal edema in Adriamycin-induced nephropathy via regulating AQP2 and miR-92b.
Le texte complet de cet article est disponible en PDF.Keywords : Zhen-wu-tang, Nephrotic syndrome, MicroRNA, Aquaporin, Edema
Plan
Vol 109
P. 1296-1305 - janvier 2019 Retour au numéro
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