Docosahexaenoic acid-flurbiprofen combination ameliorates metaflammation in rats fed on high-carbohydrate high-fat diet - 09/12/18
, Ghada M. Al-Ashmawy 1 , Asmaa A. Kamel 1, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 9 |
| Iconographies | 8 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | DHA-flurbiprofen combination protected against obesity-associated metaflammation. |
• | DHA-flurbiprofen combination improved insulin resistance and dyslipidemia. |
• | DHA-flurbiprofen combination corrected deterioration of adipose tissue morphology. |
• | DHA and flurbiprofen modulated AMPK and NF-κB expression in adipose tissue. |
• | DHA-flurbiprofen combination elevated adipose tissue RvD1 and reduced plasma leptin. |
Abstract |
Objective |
Potential benefits of combining docosahexaenoic acid (DHA), an omega-3 fatty acid with flurbiprofen (Flu), a non-steroidal anti-inflammatory drug in ameliorating obesity remain to be elucidated. This study aimed to investigate the possible protective effects of DHA and Flu, either alone or in combination, against obesity-induced metaflammation and to clarify the underlying molecular mechanisms.
Methods |
Seventy-five male Wistar rats were divided into five groups: normal diet (ND) group, high-carbohydrate high-fat diet (HCHFD) control group, DHA group (HCHFD + 200 mg/kg DHA), Flu group (HCHFD + 10 mg/kg Flu), and DHA + Flu group (HCHFD + DHA + Flu). Treatments were administered orally daily for 8 consecutive weeks, parallel with the start of diets.
Results |
Plasma levels of glucose, insulin, and TGs were significantly reduced in DHA, Flu, and DHA + Flu treated groups, while HDL-C concentrations were significantly elevated in the same groups, compared to HCHFD control group. Only Flu and DHA + Flu groups showed a significant decrease in plasma levels of leptin, TC, and LDL-C, relative to HCHFD control group. Concentrations of phosphorylated adenosine monophosphate-activated protein kinase (pAMPK) and resolvin D1 (RvD1) in epididymal adipose tissue (EAT) were significantly increased in the three treated groups, compared with HCHFD control group. Expression of AMPK-α1 subunit in EAT was significantly increased, whereas expression of nuclear factor kappa B (NF-κB) was significantly decreased in EAT of the three treated groups, relative to HCHFD control group.
Conclusions |
Docosahexaenoic acid-flurbiprofen combination showed an ameliorative effect on obesity-associated metaflammation and its consequences in rats.
Le texte complet de cet article est disponible en PDF.Keywords : Metaflammation, Obesity, DHA, Flurbiprofen, AMPK, Resolvin D1
Plan
Vol 109
P. 233-241 - janvier 2019 Retour au numéro
Article précédent
- Upregulation of circular RNA circ_0001721 predicts unfavorable prognosis in osteosarcoma and facilitates cell progression via sponging miR-569 and miR-599
- Liandi Li, Ling Guo, Guohua Yin, Guanghao Yu, Yanjing Zhao, Yanming Pan
- DPP-4 inhibitor impedes lipopolysaccharide-induced osteoclast formation and bone resorption in vivo
- Masahiko Ishida, Wei-Ren Shen, Keisuke Kimura, Akiko Kishikawa, Kazuhiro Shima, Saika Ogawa, Jiawei Qi, Fumitoshi Ohori, Takahiro Noguchi, Aseel Marahleh, Hideki Kitaura
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?