Investigation of the effects of naringin on intestinal ischemia reperfusion model at the ultrastructural and biochemical level - 09/12/18
, A. Cerkezkayabekir c, F. Sanal c, M. Inan dBienvenue sur EM-consulte, la référence des professionnels de santé.
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Graphical abstract |
The reactive oxygen species (ROS) and reactive nitrogen species (RNS) increase during the reperfusion in intestinal I/R. Cellular defenses developed against the free radical damage ensures the removal of radicals from the medium via enzymatic and non-enzymatic processes. Naringin is a bioflavonoid and polyphenolic compound that exists in many citrus fruits. It has biological and pharmacological features and antioxidant, antiulcer, anti-apoptotic, hepatoprotective, anti-diabetic, vascular dilatator and anti-inflammatory effects. Naringin provided limited healing at the ultrastructural level but also affected recovery within antioxidant parameters.
Highlights |
• | Naringin reversed cellular damage due to ischemia-reperfusion in the gut. |
• | Light and electron microscopic observations, SOD, Gpx and CAT activities as the indicators of oxidant stress were measured for tissue damage. |
• | Light microscopic evaluation was determined by Chiu score. |
• | Naringin achieved limited ultrastructural improvement, but also affected recovery in antioxidant parameters. |
Abstract |
We aimed to evaluate the ultrastructural effect of reversing cellular damage, occurring in rats due to ischemia-reperfusion (I/R) in the intestine, with naringin implementation through biochemical parameters. Rats were divided the sham/control, I/R and the naringin groups (n = 7). For I/R group, 120 min of ischemia and 120 min of reperfusion was applied to the superior mesenteric artery. In the naringin group, after 120 min, 50 mg/kg naringin was implemented, and then 120 min of reperfusion was applied. Morphological evaluation was performed via Chiu score and electron microscopy. The antioxidant parameters were examined. Chiu score in I/R (p < 0.01) and naringin (p < 0.05) groups were higher than the sham/control group. In ultrastructural level some irregularity were observed in I/R group. Although it decreased in the naringin group, the damage was observed to continue. Malondialdehyde (MDA) amount and Superoxide dismutase activity (SOD) in I/R group were higher in comparison to the sham/control group (p < 0.01), while glutathione peroxidase activity (Gpx) was found to be lower (p < 0.01). SOD (p < 0.05) and MDA (p < 0.01) were decreased by naringin group. Gpx was decreased in I/R group compared to sham/control group (p < 0.01) and elevated due to naringin administration (p < 0.05). Catalase activity was observed to decrease in the naringin group compared to control and I/R groups (p < 0.01). It was determined that naringin provided limited healing at the ultrastructural level but also effected recovery within antioxidant parameters.
Le texte complet de cet article est disponible en PDF.Abbreviations : CAT, Gpx, SOD, I/R, ROS, RNS, SMA, MDA
Keywords : Ischemia-reperfusion, Intestine, Naringin, Malondialdehyde, Antioxidant enzymes
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Vol 109
P. 345-350 - janvier 2019 Retour au numéro
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