Gender-specific effects of Notch3 in cardiac phenotype after moderate physical training - 25/12/18
Résumé |
Background |
Studies in mice have shown that the Notch3 protein plays a major role in the functional and structural plasticity of resistance arteries, angiogenesis, and contributes to the adaptation of these vessels to variations in loading conditions.
Purpose |
Our aim was to determine whether gender is a factor of the cardiac phenotype of Notch3 KO mice in the basal state and in response to a volume overload.
Methods |
Two-month-old male and female C57Bl/6J WT and KO Notch3 mice were used in a moderate training protocol on treadmill with increasing speed, in order to mimic the effort induced during cardiac rehabilitation.
Results |
In basal conditions, KO mice are characterized by a low expression of VEGFR2 mRNA, leading to an arteriolar rarefaction (P<0.0002) without changes in capillary density regardless of gender. This results to a fibrosis in KO mice. However, gender differences are observed since males display cardiac hypertrophy (P<0.0001) while females have a decreased shortening fraction (P<0.0001).
In training conditions, expression of sFlt1 mRNA increased in female Notch3 KO mice. Physical training increased the capillary density only in WT males (P<0.0332) whereas, it reduced the capillary density (P<0.0021) and induced cardiac fibrosis (P<0.0021) in Notch3 KO females. In addition, physical training reversed cardiac hypertrophy (P<0.0332) in male Notch3 KO, but has no major effect in the cardiac function of female Notch3 KO.
Conclusion |
This work highlights for the first time the gender-specific effects of Notch3 in the heart phenotype under control conditions and after moderate physical training.
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Vol 11 - N° 1
P. 47 - janvier 2019 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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