Molecular epidemiology of environmental Cryptococcus species isolates based on amplified fragment length polymorphism - 29/12/18
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Abstract |
Objective |
Cryptococcosis is a major opportunistic fungal infection caused by members of the genus Cryptococcus, mainly those belonging to the Cryptococcus neoformans/Cryptococcus gattii species complexes. Here, we report a comprehensive molecular epidemiological study of the environmental distribution of Cryptococcus isolates in Shiraz, Iran with review of litreature.
Method |
A total of 406 samples were obtained from Eucalyptus trees and 139 samples from pigeon droppings. Cryptococcus species identification and genotyping were performed by amplified fragment length polymorphism (AFLP) fingerprinting sequencing and sequencing of the ITS rDNA region.
Results |
Majority of the isolates belonged to the Naganishia taxon (n=69) including N. albida (formerly C. albidus, n=62), N. globosa (formerly C. saitoi, n=4), N. adeliensis (formerly C. adeliensis, n=2), N. diffluens (formerly C. diffluens, n=1), and the identified C. neoformans isolates (n=25) belonged to genotype AFLP1/VNI (n=22) and AFLP1B/VNII (n=3).
Conclusion |
More research efforts should be employed to isolate C. gattii species complex from environmental niches in Iran and provide additional evidence related to novel molecular types.
Le texte complet de cet article est disponible en PDF.Keywords : Cryptococcus species, Amplified Fragment Length Polymorphism (AFLP) fingerprinting, Environmental sampling, Iran
Plan
Vol 28 - N° 4
P. 599-605 - décembre 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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