Low frequency of itraconazole resistance found among Aspergillus fumigatus originating from poultry farms in Southwest Poland - 05/01/19

Doi : 10.1016/j.mycmed.2018.12.005

U. Nawrot ^a,^⁎ , A. Wieliczko ^b, K. Włodarczyk ^a, E. Kurzyk ^c, A. Brillowska-Dąbrowska ^c
^a Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Medical University of Wrocław, Borowska 211A, 50-556 Wrocław, Poland
^b Department of Epizootiology and Clinic of Birds and Exotic Animals, The Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland
^c Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, Gdańsk, Poland

^⁎Corresponding author.

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Abstract

Aim

The aim of this study was to investigate the collection of avian Aspergillus fumigatus isolates for the presence of triazole resistance.

Material and method

The study was performed on 60 A. fumigatus isolates cultured from lung tissue samples from chicken (25), geese (17), turkeys (13) and ducks (5). The samples were obtained from 40 different farms located in the Southwest Poland and were collected in the period of September 2015 to November 2016. The EUCAST microdilution method, with the use of three concentrations of itraconazole (ITR) (1, 0.5, and 0.25mg/L), was used to screen the susceptibility of all isolates. Additionally, the selected 20 isolates were tested with eleven concentrations ranging 0.015–16mg/L of ITR, voriconazole, posaconazole and isavuconazole.

Results

Most tested isolates (59/60) were susceptible to ITR (MIC≤0.5mg/L). One isolate showed elevated MIC for ITR (16mg/L), as well as voriconazole (4mg/L), izavuconazole (4mg/L), and posaconazole (0.5mg/L). This isolate was identified on the basis of DNA analysis as A. fumigatus carrying TR34/L98H mutation. All of the ITR-susceptible isolates under study were also susceptible to other triazoles.

Conclusion

Obtained results indicated a low frequency (1.6%) of A. fumigatus resistant to triazoles among avian isolates from the Southwest regions of Poland.

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Keywords : Aspergillosis, Aspergillus fumigatus, Resistance to triazoles, Domesticated birds

Plan

Introduction

Material and methods

Strains and study design

Antifungal susceptibility testing

Validation of the detection of resistant isolates in polyclonal cultures

Molecular tests used to identify and to determine mechanism of resistance to triazoles

Results

Discussion

Disclosure of interest

☆	The study was in parts publish as poster presentation on 8th Trends in Medical Mycology, Belgrad, 6–9 October 2017, Mycoses 2017, 60, S 2, 62 P017.