Lycium barbarum polysaccharides protects H9c2 cells from hypoxia-induced injury by down-regulation of miR-122 - 06/01/19
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Graphical abstract |
Highlights |
• | LBPs protects H9c2 cells against hypoxia-induced injury. |
• | miR-122 is low expressed by LBPs treatment. |
• | The protection of LBPs in hypoxia-injured cells are attenuated by pre-miR-122. |
• | LBPs activates MEK/ERK and AMPK pathways, while pre-miR-122 deactivates them. |
Abstract |
Background |
Lycium barbarum polysaccharides (LBPs) are major ingredients of fructus lycii, which have multiple pharmacological activities, such as antioxidant, neuroprotective, and anti-inflammatory activities. This study attempted to reveal the potential of LBPs in hypoxia-injured H9c2 cells and the possible underlying mechanisms.
Methods |
H9c2 cells were treated by 300 μg/mL LBPs for 24 h upon hypoxia. Subsequently, the changes in cell viability, migration and apoptosis were evaluated. pre-miR-122 or miR-122 sponge was transfected into H9c2 cells to investigate whether miR-122 was involved in the mechanisms of LBPs’ action. Besides, an animal model of myocardial infarction (MI) was established, and the in vivo effects of LBPs were further investigated.
Results |
LBPs increased cell viability, down-regulated p53, p21 and p16 protein expressions, improved migration, and repressed apoptosis in hypoxia-injured H9c2 cells. miR-122 was highly expressed in response to hypoxia, while was down-regulated by addition of LBPs. The protective actions of LBPs in hypoxia-injured H9c2 cells were attenuated by miR-122 overexpression, while were accelerated by miR-122 suppression. Also, LBPs-induced the activation of MEK/ERK and AMPK signaling pathways were attenuated by miR-122 overexpression, and were accelerated by miR-122 suppression. in vivo investigation revealed that, MI rats administrated with LBPs decreased infarct size and improved cardiac function via down-regulation of miR-122.
Conclusion |
LBPs exhibited in vitro and in vivo cardioprotective activities via down-regulating miR-122. LBPs may have potential for the treatment of acute myocardial infarction (AMI).
Le texte complet de cet article est disponible en PDF.Keywords : Lycium barbarum polysaccharides, Acute myocardial infarction, H9c2 cell, Hypoxia, miR-122
Plan
Vol 110
P. 20-28 - février 2019 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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