Therapeutic evaluation of acupoint stimulation with needle-scapelon on rat model of degenerative cervical intervertebral discs - 06/01/19
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Highlights |
• | Acupoint stimulation with needle-scapelon increased type I collagen production. |
• | Acupoint stimulation decreased type II collagen production. |
• | Acupoint stimulation around neck could inhibit intervertebral disc degeneration. |
Abstract |
Cervical spondylosis (CS), which is resulted from degeneration of cervical intervertebral disc, is a common disease seriously threatening human health and quality of life. However, there is still no effective clinic strategies for the treatment of this disease. The acupoint stimulation with needle-scalpel is a widely used approach to treat orthopedic diseases. In the present study, we evaluated the therapeutic effects of acupoint stimulation around neck with needle-scalpel on delaying the degeneration of cervical intervertebral discs and hopefully provided an approach for the precaution and early intervention of CS. We firstly established a rat model of CS by cervical static-dynamic imbalance to mimics disc degeneration and then stimulated the acupoints around neck with needle-scalpel. The cervical intervertebral disc samples were collected to measure type I and II collagen by quantitative PCR (qPCR), immunohistochemistry, and western blot. The changes in micro-structure and ultra-structure of nucleus pulposus were analyzed under the optical microscope and electron microscope respectively. Acupoint stimulation with needle-scapelon increased type I collagen production and decreased type II collagen production, and improved the micro-structure and ultra-structure of nucleus pulposus. Our results suggest that acupoint stimulation around neck with needle-scapelon could inhibit intervertebral disc degeneration through modulating the extracellular matrix collagen system and improving the changed structure of nucleus pulposus.
Le texte complet de cet article est disponible en PDF.Abbreviations : CS, qPCR, TCM, SSL, ISL, HE, PBST, ECM, SDS-PAGE
Keywords : Needle-scalpel, Cervical spondylosis, Cervical intervertebral disc degeneration, Collagen, Ultra-structure
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Vol 110
P. 677-684 - février 2019 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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