Involvement of stromal cell-derived factor-1α (SDF-1α), stem cell factor (SCF), fractalkine (FKN) and VEGF in TSG protection against intimal hyperplasia in rat balloon injury - 06/01/19
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Graphical abstract |
Highlights |
• | TSG inhibited intimal hyperplasia in rat common carotid artery balloon injury. |
• | TSG reduced the expression of SCF/c-kit, SDF-1α/CXCR4 and FKN/CX3CR1 axes. |
• | TSG enhanced re-endothelialization through increasing VEGF levels and CD34 cells. |
Abstract |
Background |
Intimal hyperplasia is the major therapeutic concern after percutaneous coronary intervention. The aim of this study is to investigate effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D glucoside (TSG) on intimal hyperplasia and the underling mechanisms through attenuating the expressions of stromal cell-derived factor-1α (SDF-1α)/CXCR4, stem cell factor (SCF)/c-kit and fractalkine (FKN)/CX3CR1, and through promoting re-endothelialization with vascular endothelial growth factor (VEGF).
Method |
Rats were operated with carotid artery balloon injury. The treatment groups were gavaged with 50 and 100 mg/kg/d of TSG. After 10 days of treatment, carotid artery pathological changes were evaluated by histology. Serum levels of SDF-1α, SCF, FKN and VEGF were detected by enzyme linked immunosorbent assay. The protein expressions of the receptors c-kit, CXCR4, CX3CR1, as well as CD34 and proliferating cell nuclear antigen (PCNA) were detected by immunochemistry.
Results |
TSG dose-dependently inhibited balloon injury-induced intimal hyperplasia, as evidenced by reducing neointima area (NIA), neointima area/media area (NIA/MA), neointima area/internal elastic area (NIA/IELA), and by decreasing the protein expression of PCNA. TSG reduced serum levels of SDF-1α, SCF and FKN, and it also decreased the expressions of the corresponding receptors c-kit, CXCR4, CX3CR1 in neointima. Importantly, the level of VEGF in peripheral blood and the expression of CD34 in vascular walls were increased to promote re-endothelialization.
Conclusions |
This study clearly demonstrated that TSG was effective in inhibiting intimal hyperplasia, and this effect was mediated, at least in part, through the SCF/c-kit, SDF-1α/CXCR4 and FKN/CX3CR1 axes. Importantly, TSG could increase VEGF and CD34 to promote endothelial repair.
Le texte complet de cet article est disponible en PDF.Abbreviations : TSG, SCF, PCI, SDF-1α, FKN, VEGF, ELISA, PCNA, VSMCs, HUVECs, ECs, NIA, MA, IELA, H&E
Keywords : Balloon injury, TSG, SCF/c-kit, SDF-1α/CXCR4, FKN/CX3CR1, Intimal hyperplasia
Plan
Vol 110
P. 887-894 - février 2019 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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