Inhibitory effects of Morus nigra L. (Moraceae) against local paw edema and mechanical hypernociception induced by Bothrops jararacussu snake venom in mice - 16/02/19
, Juliana Mikaely Dias Soares a , Helder Anderson Lima Silva b , Carlos Wagner de Souza Wanderley c , Celuane Alves Moura a , Raimundo Gonçalves de Oliveira-Junior a , Ana Paula de Oliveira a , Larissa Araújo Rolim a , Emmanoel Vilaça Costa d , Jackson Roberto Guedes da Silva Almeida a , Helinando Pequeno de Oliveira a, e , Raimundo Campos Palheta-Junior a, b, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Graphical abstract |
Highlights |
• | Morus nigra decreased paw edema and hypernociception induced by Bothrops venon. |
• | The anti-inflammatory activity of Morus nigra, gets retained in the drug carrier. |
• | Morus nigra reduces paw edema and leucocyte migration induced by carrageenan. |
• | Morus nigra effects involve the modulation of serotonin and bradykinin actions. |
Abstract |
Background |
Bothropic venoms cause intense local damage, pain, edema, and myonecrosis. Morus nigra L. (Moraceae) has several uses in folk medicine and can be a promising candidate for the treatment of several inflammatory disorders.
Hypothesis/Purpose |
The present study aims to evaluate the anti-inflammatory and antinociceptive effects of the ethanolic extract of Morus nigra L. (Mn-EtOH) on paw lesions induced by Bothrops jararacussu snake venom (BjcuV) in mice.
Methods |
UV–vis absorption of BjcuV was evaluated. A phytochemical study was performed, which led to the isolation and characterization of three compounds. These compounds were identified using spectrometric methods, namely LC–MS and NMR (1D and 2D), followed by the validation of their spectra with the data available in the literature. Further, the flavonoids i.e. rutin and quercetin (chemical markers of M. nigra), Mn-EtOH or Mn-EtOH-encapsulated electrospun fibers of Eudragit L100 (FB/Mn-EtOH), and Mn-EtOH-encapsulated microparticles of Eudragit L100 (MP/Mn-EtOH) were evaluated, in paw edema test induced by BjcuV.
Results |
UV–vis spectra showed the presence of phospholipases A2 as component of BjcuV. The chemical examination resulted in the isolation of β-sitosterol, quercetin-3-O-glucopyranoside, and kaempferol-3-O-glucopyranoside. Mn-EtOH, FB/Mn-EtOH, MP/Mn-EtOH, rutin, and quercetin reduced the local edema induced by BjcuV. The Mn-EtOH also prevented edema provoked by serotonin and bradykinin. Moreover, it reduced paw edema and peritoneal leukocyte infiltration induced by carrageenan, and decreased the mechanical hypernociception of BjcuV. Mn-EtOH exerted anti-inflammatory and antinociceptive effects, possibly by the inhibition of leukocyte migration and the modulation of serotonin and bradykinin actions. This anti-inflammatory activity was maintained even upon incorporation of the M. nigra extract into the drug delivery systems (i.e., Mn-EtOH-encapsulated FBs and MPs of Eudragit L100).
Conclusion |
These results reinforce the therapeutic potential of M. nigra in the treatment of inflammatory conditions, in addition to, its role as a complementary treatment of snakebites.
Le texte complet de cet article est disponible en PDF.Abbreviations : Mn-EtOH, BjcuV, LC–MS, NMR, FB/Mn-EtOH, MP/Mn-EtOH, NSAIDs, DDSs, PGE2, SEM, FT-IR, 5-HT, COX
Keywords : Inflammation, Morus nigra, Bothrops jararacussu, Edema, Electrospun fibers, Pain
Plan
Vol 111
P. 1046-1056 - mars 2019 Retour au numéro
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