Protective effects of p-coumaric acid against oxidant and hyperlipidemia-an in vitro and in vivo evaluation - 16/02/19
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Graphical abstract |
Highlights |
• | p-Coumaric acid had radical scavenging ability and ferric ion reducing antioxidant power. |
• | p-Coumaric acid inhibited AAPH-induced ROS generation and LDH release. |
• | p-Coumaric acid increased expression of antioxidant genes in vivo. |
• | p-Coumaric acid promoted the recovery of hyperlipidemia steatohepatitis in vivo. |
Abstract |
Dietary phenols are antioxidants with diverse physiological functions that are beneficial for human health. The objective of this research work was to investigate antioxidant activity of p-coumaric acid (p-CA) using four in vitro methods, the protective effects against oxidative stress in PC12 cells, and hypolipidemic effects on High fat-diet (HFD) mice model. The p-CA exhibited moderate antioxidant activity in the selected in vitro assay. The highest chelating activity of p-CA at 50 μg/mL was found to be 52.22%. Pretreatment with p-CA significantly enhanced cell viability of PC12 cell and suppressed AAPH-induced intracellular ROS generation and AAPH-induced LDH release. The hypolipidemic effects of p-CA (100 mg/kg BW) was directly linked to the increased expression of nuclear factor erythroid 2-related factor (Nrf2) by 2.0-fold, Glutathione peroxidase (Gpx) by 3.8-fold, Superoxide dismutase (SOD-1) by 1.6-fold, Heme oxygenase (HO-1) by 1.72-fold and NAD(P)H Quinone Dehydrogenase 1 (NQO-1) by 1.5-fold compared with HFD group. In addition to these effects, p-CA decreased total cholesterol and atherosclerosis index levels, and increased catalase (CAT) level in serum, total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) levels in liver as compared HFD group. Administration of p-CA also promoted the recovery of hyperlipidemia steatohepatitis in mice by ameliorating lipid peroxidation. These results suggested that p-CA is a potent antioxidant with potential therapeutic efficacy for treating hyperlipidemia symptoms.
Le texte complet de cet article est disponible en PDF.Keywords : p-Courmaric acid, Antioxidant activity, Oxidative stress, PC12 cells, High fat-diet mice model
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Vol 111
P. 579-587 - mars 2019 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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