Small RNA-sequencing identified the potential roles of neuron differentiation and MAPK signaling pathway in dilated cardiomyopathy - 02/04/19

Doi : 10.1016/j.biopha.2019.108826

Guangyong Huang ^a,^⁎,^{^{1
The authors contributed equally to this work.}} , Miaomiao Cao ^a,^{^{1
The authors contributed equally to this work.}}, Zhiqi Huang ^b,^{^{1
The authors contributed equally to this work.}}, Youzhang Xiang ^c, Jingwen Liu ^a, Yuehai Wang ^a, Jing Wang ^c, Wenbo Yang ^d,^⁎
^a Department of Cardiology, Liaocheng People’s Hospital of Shandong University, Liaocheng Clinical School of Taishan Medical University. Liaocheng, China
^b Department of Geriatric Medicine, Civil Aviation General Hospital, Beijing, China
^c Shandong Institute for Endemic Disease Control, Jinan, Shandong, China
^d Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

^⁎Corresponding author at: Department of Cardiology, Liaocheng People’s Hospital of Shandong University, Liaocheng Clinical School of Taishan Medical University. No 67, West Dongchang Road, Liaocheng, 252000, China.Department of CardiologyLiaocheng People’s Hospital of Shandong UniversityNo 67West Dongchang RoadLiaocheng252000China^⁎⁎Corresponding author at: Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. No 197, Ruijin 2nd Road, Shanghai, 200025, China.Department of CardiologyRuijin HospitalShanghai Jiao Tong University School of MedicineNo 197Ruijin 2nd RoadShanghai200025China

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Highlights

•	Small RNA-sequencing was performed to detect circulating miRNAs in DCM patients.
•	Forty-eight dysregulated circulating miRNAs were detected in DCM patients.
•	Neuron differentiation was the core biological process in DCM.
•	MAPK signaling pathway was the hub pathway in DCM.

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Abstract

Dilated cardiomyopathy is a severe disease characterized by ventricular enlargement and subsequent cardiac dysfunction. MiRNAs plays multiple roles in cardiovascular disease. However, diagnosis values and therapeutic effects of miRNAs in dilated cardiomyopathy are yet poorly understood. In the present study, small RNA-sequencing was employed to identify dysregulated circulating miRNAs in DCM patients compared with healthy controls. A total of 48 dysregulated miRNAs were detected, and 7198 mRNAs, the intersection of predicted mRNAs from both Miranda database and RNAhybrid database, were identified as the target mRNAs of these dysregulated miRNAs. Bioinformatics analysis was performed to identify the potential effects of these dysregulated miRNAs in dilated cardiomyopathy. GO analysis and GO-Tree analysis disclosed that neuron differentiation was potentially the core biological process associated with dilated cardiomyopathy. KEGG analysis and Pathway-Act network showed that mitogen-activated protein kinase (MAPK) signaling pathway was the hub pathway in dilated cardiomyopathy. The dysregulated miRNAs and related target mRNAs in neuron differentiation process and MAPK signaling pathway were also presented in the study. In conclusion, forty-eight dysregulated miRNAs were identified by small RNA-sequencing. Bioinformatics analysis suggested these miRNAs might be involved in the pathogenesis of dilated cardiomyopathy via regulating neuron differentiation process and MAPK signaling pathway.

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Keywords : Small RNA-sequencing, Circulating microRNAs, Dilated cardiomyopathy, Neuron differentiation, MAPK signaling pathway