Susceptibility profile of Candida rugosa (Diutina rugosa) against antifungals and compounds of essential oils - 05/04/19

Doi : 10.1016/j.mycmed.2019.03.002

A. Ludwig ^a, F.P.K. de Jesus ^a, V. Dutra ^b, S.L. Cândido ^b, S.H. Alves ^c, J.M. Santurio ^a,^⁎
^a Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Campus UFSM, Prédio 20, Sala 4139, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil
^b Programa de Pós graduação em Ciências Veterinárias, Universidade Federal do Mato Grosso, 97105-900 Cuiabá, Brazil
^c Laboratório de Pesquisas Micológicas, Programa de Pós graduação em Ciências Farmacêuticas, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, Brazil

^⁎Corresponding author.

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Abstract

Candida rugosa (recently reclassified Diutina rugosa) is an emerging pathogen affecting humans and animals. Candida resistance to existing drugs is an important factor to be monitored, as well as the need of researching alternatives to conventional antifungals. Here, we evaluated the in vitro effects of some antifungals and major components of essential oils by the broth microdilution method (CLSI M27-A3) against fifteen C. rugosa strains from animals isolated and molecular identificated. The results showed MIC₉₀ of: 0.125μg/mL to ketoconazole and voriconazole, 0.25μg/mL to micafungin, 0.5μg/mL to anidulafungin, 1μg/mL to caspofungin, 2μg/mL to amphotericin B, itraconazole and flucytosin, 8μg/mL to fluconazole, 16μg/mL to nystatin and >128μg/mL to terbinafine. The compounds carvacrol (MIC₉₀ 320μg/mL), thimol (MIC₉₀ 320μg/mL) and cinnamaldehyde (MIC₉₀ 160μg/mL) demonstrated antifungal activity against the samples tested.