Cet article a été publié dans un numéro de la revue, cliquez ici pour y accéder
Define a precise meaning of bone by Raman spectroscopy.
The spectra intensity related to the bone constituents' concentrations.
Spectra are reflecting the rate of the healing process.
The fluorescence (no background contamination) is related to the collagen content.
Raman spectroscopy a viable investigation method for periodontal disease.
The aim of this study is to evidence the importance of the phase changes for calcified tissues evolution to the stage of “mature bone” and to define a precise meaning of bone regeneration process using Raman spectroscopy.
Materials and Methods
Reference calcium phosphates compounds and harvested bone samples from selected patients were used. Investigation method was mainly based on Raman spectroscopy.
The specific peaks for the Raman shift were traced for reference calcium phosphates compounds and bone samples, as follows: 430 – 450 cm-1 (ν2, PO43-), 955 – 960 cm-1 (immature bone, amorphous bone), 960 – 965 cm-1 (mature bone, mineral bone) and 1023 cm-1 (P2O , PPi - inorganic pyrophosphate). Depending on CO quantity, its florescence peak is more or less stronger. The Raman shift corresponding to collagen proteins belongs to 800 – 900 cm−1 interval and it is the most relevant sector for spectra curvature.
The spectra intensity variation related to the specific bone constituents' concentrations, before and after healing, is reflecting the rate of the healing process mostly by the changes that occurred for the calcified tissues. Moreover, the bone sample fluorescence is related to the collagen content, enabling the quantification of healing process.
Therefore, since a complete evaluation of the processed spectra offers quantitative information for bone samples, Raman spectroscopy can be considered a viable investigation method (even forensics) for periodontal disease and bone quality assessment.Le texte complet de cet article est disponible en PDF.
Keywords : Raman spectroscopy, Periodontal disease, Calcium phosphates, Bone augmentation, Bone tissue analysis